摘要
目的:比较3种检测肺炎支原体(Mp)的方法,寻求更加适合临床检测要求的检测手段。方法:选择148例社区获得性呼吸道感染患者,留取痰或者咽拭子、血清标本,分别经培养、培养-PCR和血清IgM抗体检测,对检测结果进行分析。结果:3种方法检测Mp的阳性率分别为2.70%、6.76%和11.49%,经统计学检验,培养法与血清学阳性率、培养-PCR法与培养法阳性率间差异均有统计学意义(均P<0.01),培养-PCR法与血清学阳性率间差异无统计学意义(P>0.05)。结论:培养-PCR法和血清学检测是肺炎支原体病原学诊断的有效手段,二者联合使用对于提高肺炎支原体感染诊断有重要帮助。
Objective: To compare three methods for detecting the Mycoplasma pneumonia, to explore a method better fitting the clinical demands. Methods: 148 patients with community-acquired respiratory tract infection were selected. Their sputum samples, throat swabs and blood serums were assembled to detect Mp-IgM antibodies through methods of cultivation, culture-PCR and serological test. The results were analyzed. Results: The positive rates of three methods for detecting Mp were 2,70%, 6,76% and 1 L49% respectively, there were statistical differences between cultivation and serological test, as well as euhure-PCR and cultivation (all P〈0.01). There were no statistical differences between culture-PCR and serological test (P〉0.05). Conclusion: Cuhure-PCR and serological test are both effective methods for detecting Myeoplasma pneumonia. Combination of the two methods can significantly improve the diagnosis of Mycoplasma pneumonia.
出处
《中国医药导报》
CAS
2010年第25期5-6,共2页
China Medical Herald