逆胶束体系中纤维素酶解特性研究

Enzymatic Hydrolysis of Cellulose in Reverse Micelles

通过采用几种不同属性类型的表面活性剂构建逆胶束体系,以羧甲基纤维素为底物,研究了纤维素酶解的特性.通过电导率的测量,确定由CTAB、SDS、Tween-80和鼠李糖脂构造的逆胶束体系的最大增溶水量W0(W0=[H2O]/[SA])分别为15.2、20.1、2.3和40.3.在此条件下,考察了不同表面活性剂浓度和不同纤维素酶用量对逆胶束体系酶解行为的影响,并与水溶液体系的酶解反应进行对比.结果表明,单位底物条件下,纤维素酶用量为0.15 FPU/g,CTAB、SDS、Tween-80和鼠李糖脂浓度均为1 cmc时,逆胶束体系酶解产量最高,其中鼠李糖脂体系产量可达198.03 mg,分别比CTAB、SDS和Tween-80体系高了10.89%、31.09%和45.30%.CTAB、SDS、Tween-80和鼠李糖脂浓度为1 cmc时,其逆胶束体系酶解产量比水溶液体系分别高了34.36%、21.24%、11.44%和34.62%.在最佳表面活性剂浓度条件下单位底物酶用量为5 FPU时,逆胶束体系酶解反应从经济和糖产量方面考虑均为最适合.生物表面活性剂鼠李糖脂体系的产量高于3种化学表面活性剂体系,说明生物表面活性剂在构建逆胶束体系及增强逆胶束稳定性能上具有特殊的潜力.

Several types of surfactants were adopted to construct reverse micelles,in order to investigate the characteristics of cellulose hydrolysis,we used the carboxymethyl cellulose as substrate.The electrical conductivity was measured to determine the maximum water solubilization W0（W0 =[H2 O]/[SA]）of CTAB,SDS,Tween-80 and rhamnolipid reverse micellar systems were 15.2,20.1,2.3 and 40.3.In this condition we studied the effects of surfactants concentrations and cellulose dosage on the enzymatic hydrolysis of reverse micelle,and compared with aqueous systems.It was shown by the results that when the cellulose dosage was 0.15 FPU /g substrate,the maximum yield of reducing sugar in reverse micelles was obtained at 1 cmc of CTAB,SDS,Tween-80 and rhamnolipid,in which the rhamnolipid yield was the highest of 198.03 mg substrate.When the concentrations of CTAB,SDS,Tween-80 and rhamnolipid were 1 cmc,the productions of reverse micelles systems were higher than that of aqueous systems of 34.36%,21.24%,11.44% and 34.62%.In the optimum conditions of the surfactant concentration,taking the saving cost and sugar yield into consideration,the cellulose dosage of 5 FPU substrate was the most suitable.The reducing sugar＇s yield of biosurfactant rhamnolipid reverse micellar system was higher than those of three chemical surfactant systems,it was shown that the adoption of biosurfactant has technologically promising prospect in constructing reverse micelles and enhancing the stability of reverse micelles.