左旋氧氟沙星对小鼠结核分枝杆菌生长及细胞免疫的影响

Effects of levofloxacin on the growth and cyto-immunology of mycobacterium tuberculosis infected mice

目的探讨左旋氧氟沙星对小鼠结核分枝杆菌生长、特异性细胞免疫和病理改变的影响,探讨左旋氧氟沙星联合其他免疫治疗的可行性。方法 38只C57BL/6小鼠尾静脉注射H37Rv结核菌(1×106CFU/只),然后随机等分为两组,一组不作处理(对照组),另一组进行左旋氧氟沙星灌胃治疗(药物组),300mg/kg,每周灌5d,疗程28d。在治疗开始后不同时间点处死小鼠,计数肺组织结核分枝杆菌菌量、观察肺组织病理变化,测定脾细胞特异性细胞因子的产生情况。结果左旋氧氟沙星能有效抑制小鼠肺组织结核分枝杆菌的生长,与对照组比较,药物组小鼠服药期间细菌量减少100倍,停药后30d仍减少10倍,差别有统计学意义(P<0.05)。同时药物治疗能减轻肺组织病理变化的程度。药物组小鼠脾细胞分泌卡介苗(BCG)特异性细胞因子IFN-γ、TNF-α、IL-10和IL-17水平明显高于对照组(P<0.05)。结论左旋氧氟沙星能有效抑制小鼠结核分枝杆菌的生长,同时能改善肺组织的病理变化和促进小鼠脾细胞分泌特异性细胞因子。

Objective To observe the effects of levofloxacin on the growth, cyto-immunology and pathology of mycobaeterium tuberculosis infected mice and evaluate the feasibility of combining levofloxacin treatment with immune therapy. Methods All 38 mice （C57BL/6） were inoculated intravenously with 1 × 10^6 CFU mycobacterium tuberculosis （H37Rv） and randomly divided into two groups. In negative control group, mice were infected but left untreated ; The other group were treated with levofloxacin in a 300 mg/kg dose and were carried out five times weekly for 4 weeks. The severity of infection and effectiveness of treat- ment were assessed by enumeration of CFU in the spleen, gross lung lesions and determination of cytokine produced by spleen cells. Results Levofloxacin can effectively restrain the growth of mycobacterium tuberculosis and mitigate the pathology of infected mice. The levels of IFN-γ, TNF-α, IL-10 and IL-17 produced by spleen cells in treatment group were higher than control group. Conclusions Levofloxacin can restrain the growth of mycobaeterium tuberculosis in mice and improve the pathology change of lung. Levofloxacin enhance the product of specific cytokine from spleen cells.