HBeAg阳性慢性乙型肝炎患者HBV基因型和亚型与肿瘤坏死因子α的关系

Relationships between hepatitis B virus genotypes and subgenotypes with tumor necrosis factor α in HBeAg-positive chronic hepatitis B patients

目的探讨福建部分地区HBeAg阳性慢性乙型肝炎(CHB)患者HBV基因型、亚型的分布与血清肿瘤坏死因子α(TNF-α)水平及其基因启动子区-238、-308位点基因多态性的关系。方法选取福建部分地区203例HBeAg阳性CHB患者,采用HBV基因分型(B、C基因型)荧光定量PCR法检测HBV基因型或HBVS基因为直接测序法检测HBV基因型、亚型;聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)的方法检测TNF-α-238及-308位点基因多态性,酶联免疫吸附试验(ELISA)测定血清TNF-α水平,并对结果进行统计分析。结果 HBV基因型B、C分别占58.6%、41.4%,其中150例HBVS基因为直接测序,Ba亚型81例,Ce亚型69例;B基因型患者平均年龄和体重指数(BMI)小于C型(P=0.020)。HBV基因型B、C的TNF-α水平、TNF-α-238和-308位点基因多态性的分布无统计学差异(P>0.05)。结论福建部分地区HBeAg阳性CHB患者HBV基因型以B型为主,其次是C型,仅见Ba和Ce亚型;B基因型患者较年轻,BMI小于C型;但HBV基因型B、C与TNF-α水平、TNF-α-238和-308位点基因多态性可能无关。

Objective To investigate hepatitis B virus （HBV） genotypes and subgenotypes in HBeAg-positive chronic hepatitis B patients from some areas in Fujian province, and the relationships between serum levels of tumor necrosis factor α （TNF-ot） and polymorphisms in TNF-α gene promoter region at the positions of - 238 and - 308. Methods All of 203 HBeAg-positive chronic hepatitis B patients from some areas in Fujian province were recruited for research. Real-time fluorescent quantitative PCR was used to test HBV genotypes and HBV S gene sequencing was applied to determine HBV genotypes and subgeno- types. Gene polymorphisms in TNF-α promoter region at position - 238 and - 308 were determined by restriction fragment length polymorphism （RFLP） assay of PCR products, and the serum levels of TNF-α were examined by enzyme linked immunosorbent assay （ELISA）. Results were analyzed by corresponding statistics. Results HBV genotypes in all patients were B and C, accounting for 58.6% and 41.4%, respectively. And in 150 patients by HBV S gene sequencing, 81 cases were subgenotype Ba and 69 cases were subgenotype Ce. The average age was younger and the mean body mass index （BMI） was lower in patients with genotype B than genotype C （P = 0. 020）. The levels of TNF-α, polymorphisms in TNF-ot promoter at position -238 and -308 were not significantly associated with HBV genotype B and C （P 〉 0.05）. Conclusions The major popular genotypes of HBV are B and then C, but only Ba and Ce subgenotype were found in some areas in Fujian province. Patients with genotype B may be younger and with lower BMI than those with genotype C. But HBV genotype B and C may be not associated with the levels of TNF-α and polymorphisms in TNF-α promoter at position -238 and -308.