摘要
利用Genbank中公布的东方百合"元帅"查尔酮合酶cDNA序列,从东方百合"元帅"花瓣中扩增CHS1的cDNA开放阅读框,正向插入植物表达载体pBI121,并采用液氮冻融法将其转化到根癌农杆菌EHA105中。结果表明:经酶切和菌液PCR分析鉴定,获得与预期大小相符的1 200 bp片段,得到含有目的片段表达载体pBI121-CHS1的农杆菌菌株,可用于新铁炮百合的遗传转化。
In this article,CHS1 cDNA ORF was cloned from flower petals of ＇Acapulco＇ according to the Lilium ＇Acapulco＇ CHS1 cDNA sequences published by GenBank and inserted pBI121 expression vector,and then transferred into competent cells of agrohacterium tumefaciens EHA105;The digestion test and colony PCR showed that the specific expression vector designated as pBI121-CHS1 was constructed successfully.It provides a basis for the research on color transgene of Lilium Formolongi.
出处
《北方园艺》
CAS
北大核心
2010年第16期145-148,共4页
Northern Horticulture
基金
云南省教育厅重点基金资助项目(07Z10035)
