番木瓜籽脂肪酸组成的分析测定

Analysis on fatty components from the seed of papaya

参照国标GB/T14772-2008,以乙醚为溶剂,采用索氏抽提法提取番木瓜籽中的粗脂肪油,再分别以N,O-二(三甲基硅烷)乙酰胺硅酯化法和碱催化甲酯化法进行衍生化处理后,通过气相色谱/质谱联用技术分析脂肪油的组成。结果显示,番木瓜籽粗脂肪油提取量为25.5%(W/W);共鉴定出16种脂肪酸类化合物,3种非甘油脂类化合物;主要成分为棕榈酸(26.89%)、油酸(22.80%)、β-生育酚(维生素E,15.29%)、亚油酸(7.21%)、硬脂酸(5.82%)、棕榈油酸(4.16%)以及角鲨烯(4.03%)等。

Rude fat from the seed of papaya was extracted by Soxhler extractor with ethyl ether as solvent according to the Chinese Standard （GB/T 14772-2008）.After being derived by trimethylsilyl-esterified with N,O-bis （trimethylsily） acetamide and methyl esterification catalysed by base,the fatty components were analyzed by GC/MS.The results showed that the extracting quantity of rude fat oil was 25.5%（W/W）,16 fatty acids and 3 non-glycerides were obtained.The main components were palmitic acid （26.89%）,oleic acid （22.80%）,β-tocopherol（vitamin E,15.29%）,linoleic acid（7.21%）,stearic acid（5.82%）,palmitoleic acid （4.16%） and rans-squalene （4.03%）.