期刊文献+

红茶提取物体外清除自由基作用研究 被引量:9

Effect of aqueous extracts from black tea on free radical scavenging
下载PDF
导出
摘要 提取了3种名优红茶水溶性成分,采用HPLC法分析提取物中8种儿茶素单体和3种茶黄素单体的含量,测定了红茶提取物体外清除2,2’-连氨-(3-乙基苯并噻唑啉-6-磺酸)二氨盐(ABTS)、二苯代苦味肼基(DPPH)自由基的能力,并分析了红茶提取物络合亚铁离子(FIC)的能力。结果表明:3种红茶水溶性提取物中,8种儿茶素总含量顺序为英红>祁红>滇红,3种茶黄素总含量顺序为英红>滇红>祁红;3种红茶都具有较强的清除ABTS自由基、DPPH自由基能力和络合亚铁离子能力,并与红茶提取物处理浓度呈剂量依赖性关系。 Eight kinds of catechins and three kinds of theaflavins were analyzed by HPLC after water-soluble components extracted from three kinds of black tea.Then,free radical scavenging abilities including 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)radical scavenging ability,1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging ability,and ferrous-ion chelating(FIC)ability of different concentration aqueous extracts were evaluated.The results showed that the order of catechins contents was Yinghong Qihong Dianhong,and the order of theaflavins contents was Yinghong Dianhong Qihong.Three black tea aqueous extracts showed significant ABTS and DPPH radical scavenging activity and ferrous-ion chelating ability.Moreover,percent DPPH and ABTS radical scavenging abilities and ferrous-ion chelating ability of black tea aqueous extracts were dose-dependent.
出处 《广东农业科学》 CAS CSCD 北大核心 2010年第8期259-261,共3页 Guangdong Agricultural Sciences
基金 广东省农业科学院院长基金项目(201018) 广东省茶树资源创新利用重点实验室启动项目(2008A060301004)
关键词 红茶 抗氧化 儿茶素 茶黄素 自由基 black tea antioxidant catechins theaflavin free radical
  • 相关文献

参考文献10

  • 1孙世利,杨军国,张岚翠,于海宁,沈生荣.儿茶素与金属离子相互作用及其生物学意义[J].细胞生物学杂志,2007,29(2):225-228. 被引量:9
  • 2Thana P,Machmudah S,Goto M,et al.Response surface methodology to supercritical carbon dioxide extraction of astaxanthin from Haematococctts pluvidis[J].Bioresource Technologt,2008(99):3110- 3115.
  • 3Turkoglu A,Duru M E,Merean N,et al. Antioxidant and antimicrobial activities of L(u, tiporus sulphureus (Bull.)Murrill[J]. Food Chemistry, 2007(101) : 267-273.
  • 4Singh N,Rajini P S. Free radical scavenging aetMty of an aqueous extract of potato peel[J].Food Chemistry,2004(85):611- 616.
  • 5Leung L K,Su Y,Chen R,et al. Theaflavins in black tea and catechins in green tea are equally effective antioxidants[J].The Journal of Nutrition, 2001 (131) : 2248-2251.
  • 6朱玉昌,焦必宁.ABTS法体外测定果蔬类总抗氧化能力的研究进展[J].食品与发酵工业,2005,31(8):77-80. 被引量:82
  • 7Millera N J,Castelluccioa C,Tijburgb L,et al. The antioxidant properties of theflavins and their gallate esters-radical scavengers or metal chelators?[J]. Febs Letters,1996(392):40-44.
  • 8Wilgus T A,Koki A T,Zweifel B S,et al. Inhibition of cutaneous ultraviolet light B-mediated inflammation and tumor formation with topical celecoxib treatment[J].Mol Carcinog,2003,38(2):49-58.
  • 9Lin Y L,Tsai S H,Lin Shiau S Y,et al.Theaflavin-3,3- digallate from black tea blocks the nitric oxide synthase by down-regulating the activation of NF-kappaB in maerophages[J]. Eur J Pharmacol, 1999,367(2-3) : 379-388.
  • 10Yang Z,Jie G,Dong F,et al. Radical-scavenging abilities and antioxidant properties of theaflavins and their gallate esters in H2O2-mediated oxidative damage system in the HPF-1 cells[J]. Toxicology in Vitro, 2008,22 (5) : 1250-1256.

二级参考文献36

  • 1郭炳莹,程启坤.茶汤组分与金属离子的络合性能[J].茶叶科学,1991,11(2):139-144. 被引量:91
  • 2表没食子儿茶素没食子酸酯(EGCG)--锗(IV)配合物的研究.表没食子儿茶素没食子酸酯(EGCG)—锗(IV)配合物的研究[J].天然产物研究与开发,1994,6(3):35-44. 被引量:2
  • 3陈勋,于海宁,沈生荣.EGCG与锌离子互作对前列腺癌细胞PC-3生长的影响[J].茶叶科学,2006,26(3):219-224. 被引量:5
  • 4Miller N J, Rice Evans C A. A novel method for mearing an tioxidant capacity and its application to monitoring lhe antioxidant status in premature neonates[J]. Clinical Science,1993,84 : 407 - 412.
  • 5Mariken J T J Arts. A critical appraisal of the use of the antioxidant capacity (TEAC) assay in defining optimal antioxidam structures[J]. Food Chemistry,2003,80:409- 414.
  • 6Dae-Ok Kim. Antioxidant capacity of phenolic phytochcmicals from various cultivars of plums [J]. Food Chemistry,2003,81:321-326.
  • 7Robin van den Berg,Guido R M M Haenen. Applicability of an improved Trolox equivalent antioxidant capacity(TEAC).assay for evaluation of antioxidant capacity measurements of mixtures[J]. Food Chemistry, 1999,66 : 511 - 517.
  • 8Arena E, Fallico B. Evaluation of antioxidant capacity of blood orange juices as influenced by constituents, concentration process and storage[J]. Food Chemistry, 2001,74 : 423-427.
  • 9Nicholas J M, Catherine A R E. The relative contribution of ascorbic acid and phenolic antioxidants to the total antioxidant activity of orange and apple fruit juices and black currant drink[J].Food Chemistry, 1997,60(3) :331 -337.
  • 10Miller N J, Rice-Evans C A. The relative contributions of ascorbic acid and phenolic antioxidants to the total antioxidant activity of orange and apple fruit juices and blackcurrant drink[J]. Food Chemistry, 1997b ,60:331 -337.

共引文献89

同被引文献147

引证文献9

二级引证文献46

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部