免疫状态对Fv-4基因抗Friend MuLV作用的影响

Effect of Immune States on the Fv 4 Antiviral Gene Resistant to Friend Murine Leukemia Virus Infection

通过检测免疫抑制的Ｆ１小鼠和杂合子Ｆ２裸鼠对Ｆｒｉｅｎｄ小鼠白血病病毒（Ｆｒ．ＭｕＬＶ）感染的敏感性，探讨免疫状态对Ｆｖ－４基因（特别是对Ｆｖ－４基因杂合子）抗ＦｒｉｅｎｄＭｕＬＶ作用的影响。经腹腔接种Ｆｒ．ＭｕＬＶ病毒液攻击小鼠或Ｆ２裸鼠后，检查其脾脏中Ｆｒ．ＭｕＬＶ的增殖及其发病情况。结果表明，免疫抑制的Ｆ１小鼠和杂合子Ｆ１裸鼠都可被Ｆｒ．ＭｕＬＶ感染，脾脏中有病毒增殖；并且，约２／３的杂合子Ｆ２裸鼠与ＢＡＬＢ／Ｃ小鼠一样，在接种病毒后１个月内发病或死亡。说明机体的免疫状态可影响Ｆｖ－４基因，特别是Ｆｖ－４基因杂合子的抗Ｆｒ．ＭｕＬＶ作用。提示在进行基因治疗时要考虑机体的免疫状态等因素。

We have been detected the susceptibility of immune suppressive F1 mice and F2 nude mice carrying Fv 4 gene heterozygous to infection of Friend murine leukemia virus(Fr.MuLV),for investigate the effect of immune states on the Fv 4 antiviral gene resistant to Fr.MuLV infection in this paper.The F1 mice were the offspring of Fv 4 mice (Fv 4 gene homozygous)and BALB/C mice,gene type is shown as Fv 4 r/- .F2 nude mice were expected that by mating between brother and sister of F1 hybrid nude mice,which was derived that by mating Fv 4 mice and BALB/C nude mice.Fr.MuLV was injected intra abdominal to the F1 mice which had been given immune inhibitor FK506 and F2 nude mice. Result showed that Fr.MuLV could infect F2 nude mice carrying Fv 4 gene heterozygous and F1 mice which had treated with immune inhibitor FK506,and Fr.MuLV could propagated in mice spleen,and caused leukemia in the 2/3 of F2 nude mice carrying Fv 4 gene heterozygous.This result suggested the sensitivity of Fv 4 gene heterozygous mice on infection of Fr.MuLV was determined by murine ecological elements such as immune states.The antiviral effect of Fv 4 antiviral gene waw influenced by immune states of mouse.