辽宁省肾综合征出血热沙鼠苗和地鼠苗的人群抗体观察

The Immunological Effects of HFRS Mongolian gerbils Kidney Vaccine and Hamsper Kidney Vaccine

采用ＩＦＡＴ和ＭＣＰＥＮＴ法检测荧光抗体和中和抗体对肾综合征出血热（ＨＦＲＳ）沙鼠苗和地鼠苗进行免疫学效果观察。经过六次采血（即免前，基免后两周，加强免疫前，加强免疫后两周，基免后第三年，基免后第四年）检测抗体，大洼地区沙鼠苗ＩＦＡ抗体阳转率分别为６．２％（隐性感染）、１００％、５４．９％、１００％、７７．４％、７２．０％；中和抗体阳转变分别为０、７０．４％、０、７３．０％、３２．３％、３２．０％。葫芦岛地区地鼠苗ＩＦＡ抗体阳性率分别为９．９％（隐性感染）、１００％、３６．４％、１００％、７７．１％、８０．０％；中和抗体阳转率分别为０、５０％、１８％、１００％、５１．４％、４４％。表明两型疫苗均具有良好的保护性和抗体的持续性。

The purpose of this report was to observe and analyse immunological effects of the two types of inactivated vaccines against HFRS.The mongolian gerbils Kidney vaccine(type Ⅰ)was used in Dawa County,the hamsper kidney vaccine (typeⅡ)was used in Huludao City.We collected blood samples before and after basic immunization ,before and after reimmunization ,on the third and fouth year after the reimmunizatin ,using IFAT and MCPENT techniques to detect fluorecent and neutralizing antibodies.The result indicates that for type Ⅰ vaccine,the positive rate of IFA antibody is 6 2%(subclinical infection),100%、54 9%,100%,77 4%,72 0% respectively;the positive rate of neutralizing antibody is0,70 4%,0,70 3%,72 0% respectively;the positive rate of neutralizing antibody is 0,70 4%,0,70 3%,32 3%,32 0% respectively.For typeⅡ vaccine,the positive rate of IFA antibody is 9 9%(subclinical infection),100%,36 4%,100%,77 1%,80 0% respectively;the positive rate of neutralizing antibody is 0,50%,18%,100%,51 4%,44 0% respectively.Conclusion:The two types of vaccines all have good protective action and persistence of antibodies.