一氧化氮等自由基对矽肺患者损伤效应的研究

Study on Effects of Nitric Oxide and Other Free Radicals Damaging Silicosis Patients.

检测７３例矽肺患者和６０例健康对照者血浆一氧化氮（Ｐ－ＮＯ）、维生素Ｃ（Ｐ－ＶＣ）、维生素Ｅ（Ｐ－ＶＥ）、β－胡萝卜素（Ｐ－β－ＣＡＲ）、过氧化脂质（Ｐ－ＬＰＯ）及红细胞超氧化物歧化酶（Ｅ－ＳＯＤ）、过氧化氢酶（Ｅ－ＣＡＴ）、谷胱甘肽过氧化物酶（Ｅ－ＧＳＨ－ＰＸ）和过氧化脂质（Ｅ－ＬＰＯ）值的结果表明，与对照组比较，患者组Ｐ－ＶＣ、Ｐ－ＶＥ、Ｐ－β－ＣＡＲ、Ｅ－ＳＯＤ、Ｅ－ＣＡＴ、Ｅ－ＧＳＨ－ＰＸ平均值均显著降低（Ｐ＜０．００１），Ｐ－ＮＯ、Ｐ－ＬＰＯ、Ｅ－ＬＰＯ平均值均显著升高（Ｐ＜０．００１）；上述检测值与患者病程、病情、肺功能状态均有相关；逐步回归表明，患者病程与Ｐ－ＮＯ、Ｐ－ＶＥ、Ｅ－ＳＯＤ、Ｅ－ＬＰＯ值相关最为密切；提示矽肺患者体内一氧化氮和氧自由基反应病理性加剧。

To study the effects of nitric oxide and other free radicals damaging silicosis patients,we measured nitric oxide(P NO),vitamin C(P VC),vitamin E(P VE),beta carotene(P β CAR),lipoperoxides(P LPO)contents in plasma and superoxide dismutase(E SOD),catalase(E CAT),glutathione peroxidase(E GSH PX)activities and lipoperxides(E LPO)content in erythrocyte of 73 silicosis patients and 60 healthy subjects.The results showed that Compared with the control group,the average P VC,P VE,P β CAR contents and E SOD,E CAT,E GSH PX activities of the patient group significantly lowered(P<0 001),the average P NO,P LPO,E LPO contents significantly increased(P<0 001).There were relationships in all indexes and course,condition,pulmonary function of silicosis patients.The stepwise regression showed that the correlation between course,condition,pulmonary function of patients and P NO,P VE,P LPO,E LPO values was clear.The nitric oxide and oxygen free radical reactions in the body of silicosis patients were pathologically exacerbated,the balance between oxidation and antioxidation was clearly disturbed.