hIL-16 cDNA的克隆、测序及在E.coli中的表达与纯化研究

Cloning, expression and purification of human interleukin-16

在国内首次从人的外周血单个核细胞（PBMC）中克隆了人白细胞介素—16（hIL－16）cDNA，并应用一种新型的大肠杆菌表达系统一硫氧还蛋白表达系统，成功地表达了重组hIL-16。hIL-16cDNA的扩增：从人PBMC中提取总RNA，poly（T）8-12反转录cDNA，以半巢式PCR扩增出特异的DNA片段。hIL-16cDNA的克隆、测序与表达：利用E．coli表达载体pTrxFus，构建hIL－16。DNA的重组质粒，转化E．coli后，经色氨酸诱导，表达出相对分子质量（Mr）为30000的可溶性融合蛋白，表达量占菌体蛋白的30％。序列测定证实，此片段长393bp，确为hIL-16cDNA。经一步渗透压休克，即获得了高纯度的表达蛋白。

aam cDNA encwhng human interleukin-16 was amPMed by RT-PCR from total RNA Of the peripheral blood clear Cease frOIn hepatihs C virus carrier and a recombinant plasmid pTflL 16 was constructed by cloning cDNA Of ac 16 intO E. ed expresaing veltor PTrxFus (Invibogen Co. ). A soluble I hioredoaln-hll16 fhaion akin with relative molecular mass (Mr) 30 000 was expressed in E. coli GI724 habouring plashed PTffel6. The fusion Proton accoUnta for 30 % of the total protein from the bacteria. The pubbed fusion proals was obtained face inod bacteria by oslnotic shOCk. DNA seqUencing proved the cloned cDNA Of hId16 bas very high homogenicity with other reports.