打点杂交及RNA酶保护分析法检测反义HSP90基因转染细胞中反义RNA的表达

Expression of antisense RNA in HSP90 antisense RNA transfected cells determined by dot blot and RNase protection assay

目的：检测HSP90反义核酸转染细胞后反义RNA的表达。方法：采用打点杂交及RNA酶保护分析法。结果：HSP90反义核酸转染细胞AH－SGC7901，AH-SGC7901／VCR，AH－HCC7402及AH－Ec109有HSP90反义RNA的表达。结论：HSP90反义RNA在AH－SGC7901，AH－SGC7901／VCR，AH－HCC7402及AH－Ec109细胞的表达，为进一步研究HSP90在细胞生物学中的作用建立了可靠的细胞模型。

To determine the expression of antisensc RNA of HSP90 in HSP90 antisensc RNA transfected cells. Methods:Dot blot and RNase protection assay were employed. Results: AH-SGC 7901, AH-SGC 7901 / VCR, AH-HCC 74O2 and AH-Ec 109 cells transfected with HSP90 antisense RNA all expressed HSP90 antisense RNA. Conclusion:AH-SGC7901, AH-SGC 7901 / VCR, AH-HCC7402 and AH-Ec 109 express HSP90 antisense RNA. This provides a basis for the research Of the biological roles of HSP90 in the above cells.