高转移性人成骨肉瘤细胞系在裸小鼠体内的筛选和维持

Selection and maintenance of a highly metastatic subpopulation from a human osteosarcoma cell line in nude mice

目的：筛选人成骨肉瘤高转移细胞并探讨维持其高转移性状的方法。方法：采用低转移性成骨肉瘤细胞系在裸小鼠腹腔内连续传代和体外培养肺转移灶筛选高转移亚系，通过体内、外交替传代维持其高转移性状，并用流式细胞仪、染色体分析等方法，研究该高转移亚系的细胞周期、形态、增殖等生物学特性。结果：筛选所得腹水型高转移亚系自发性肺转移率达100％。在细胞形态、增殖速度、染色体数目等方面与母系有较大差别，经体内、外交替传代高转移性状得以维持，且出现较高比例的脑、骨、肌肉转移。结论：腹腔连续传代培养转移灶和体内、外交替传代是筛选和维持高转移细胞的可靠方法。

To select a highly metastahc subpopulation from a hUman osteooarcoma cell line and to establish a method for keeping its hidly methetatic character. Methods: A low metastatic Cell line SOSP-9607 was chosen as material. Anificial lung metastasis in nude face and cell culture tecboques were used to select the highly metastatic subpopulation, and its highly metaStatic character was bontalned by secondary culture in yial and in Vial. Cell growth analysis, chromosome staining and llowcytometly were employed to characterize the subpopulation. Results: The sPOntaneous lung metastasis rate of SOSP-M in nude face was 100%. Compared with SOSP-9607, SOSP-M showed different propenies in shape, growing rate, chromosome and so on. Through secondary culture in vivo and in vitro, we bontalned its metaStatic character, and observed a high rate Of metastasis in brain, bone and muscle. Conclusion: Artificial lung metastasis, cell culture and secondal culture in vivo and in vitro are reliable methods to select and maintain highly metastatic subpopulation.