磁性免疫微球的合成及其在基因重组IL-2纯化中的应用

Synthesis of immunomagnetic microsphere and its separation for recombinant human IL-2

本文在合成Fe3O4磁性材料的基础上，制备出agarose－Fe3O4磁性动体激球，并通过优化合成条件，建立了一套制备磁性微球的合成方法。将其与抗rhIL-2单克隆抗体（mAb）连接制备的免疫磁性极球一步纯化rhIL-2后，经SDS-PAGE、光密度扫描和CTLL细胞活性测定表明，纯度为93％，活性回收率为80％，比活性为2．0x109IU／g蛋白质。结果说明磁性免疫微球不失为一种简单、快速分高蛋白质的方法。

The synthesis of immunomagnetic ndcmepbere(IMMS) of monoclonal antibodies (mAb) to recombinant human IL-2(rhIL-2) as ho to Fe3O4-po ndcmephere (aMS) and separation for rblbZ from suspendon of in ed have ben in thes Paper. minaboc ndcmephere was Preped by physics wrapper encirclement. After reaction. the tie poWer menvelope in the griddle of agarose. And then, magnehc ndcmephere was coupled with anti to form rosphere. The coupling method of mAb with MMS were CNBr methods. The Iaps was ed to ho sus of inAsion . aam party of rffe-2 by one step reached 93%,specific activity 2. 0 x 106IU/mg protein, ratio of arhvity 80%.There are two main advanbo of IMMS in seaing protein. Face, acs method can separate protein from suspension.blond, it is very simple ed fast.