纳米氧化铜巯基乙胺修饰玻碳电极测定8-羟基-2’-脱氧鸟苷

Electrochemical Detection of 8-Hydroxy-2’-Deoxyguanosine Using Copper Oxide Nanocrystals/Cysteamine Film Modified Electrode

制备了纳米氧化铜/巯基乙胺/玻碳电极（nano-Cuo/CA/GCE）电化学传感器.使用扫描电子显微镜、电化学阻抗图谱和循环伏安法对此修饰电极进行了表征,应用示差脉冲伏安法测定了8-羟基-2’-脱氧鸟苷（8-OH-dG）.实验发现,8-OH-dG在nano-Cuo/CA/GCE上显示很强的电化学响应.在最佳实验条件下,测得的线性范围为0.40μM-44.0μM,检出限为0.07μM.实验结果表明此电极具有较好的抗干扰能力,并且被用于样品中8-OH-dG的测定.

Copper oxide nanocrystals （nano-CuO） was prepared and was bound to cysteamine （CA） film that was covered glassy carbon electrode （GCE）.The nano-CuO/CA/GCE was characterized with scanning electron microscopy,electrochemical impedance spectroscopy.The fabricated nano-CuO/CA/GCE sensor exhibited sensitive response to 8-OH-dG in 0.1 M PBS （pH 7.0）.The anodic peak currents were linear with the 8-OH-dG concentration over the range of 0.40μM to 44.0μM with the correlation coefficient of 0.999,and the corresponding detection limits was 0.07μM （S/N=3）.The nano-CuO/CA/GCE had good storage stability,reproducibility and anti-interference ability and could be applied to detect 8-OH-dG in samples.