提高长春花冠瘿细胞中吲哚生物碱含量的研究

Studies on the Enhancing Accumulation of Indole Alkaloids in Crown Gall Cells of Madagascar Periwinkle (Catharanthus roseus)

研究了长春花冠瘿细胞接种物年龄与接种量对悬浮培养细胞生物产量的影响，探讨了以大丽花轮枝孢菌的匀浆物为外源刺激物处理冠瘿细胞，对细胞生长和吲哚生物碱积累的作用。确立了外源刺激物产生效应的最适条件。

Influences of cell age (at inoculation) and the amount inoculated on the biological mass production of cultured cells in Catharanthus roseus (L. ) G. Don suspension were investigated. Effects of treatment by Verticillium dahliae homogenate as elicitor on the cell growth and accumulation of indole alkaloids in the crown gall cells were studied and the optimal stimulation conditions were determined.