摘要
在国内首次研究了贯叶连翘胚轴和子叶的培养方法，分别通过器官型和器官发生型途径获得了大量再生植株，部分可移栽成活。实验表明，在ＢＡ和２，４－Ｄ不同组合的ＭＳ培养基上，愈伤组织易于诱导，繁殖速度快，并且通过初步检测证明了愈伤组织中金丝桃素存在的可能性。再生植株产生极容易，繁殖系数高。
Tissue culturing of Hypericum perforatum L. from plumular axis and cotyledon explants were studied for the first time in our country. Regenerated individual plantlet could be obtained through callus or explants directly, and some of them could be transplanted successfully. On MS medium supplemented with BA and 2,4-D, the callus was easy to induce and propagated rapidly. Hypericin was possibly present in the callus by initial tests. The regenerated individual plantlets were easy to obtained and its propagate coefficient was high.
出处
《中草药》
CAS
CSCD
北大核心
1999年第2期132-134,共3页
Chinese Traditional and Herbal Drugs
关键词
贯叶连翘
组织培养
愈伤组织
植株再生
Hypericum perforatum L.
tissue culture callus
regenerate individual
hypericin
