肺癌组织中微卫星DNA的改变

The alteration of microsatellite DNA in lung cancer

目的研究微卫星ＤＮＡ在肺癌组织中的改变以及其对肺癌的诊断价值。方法应用聚合酶链反应（ＰＣＲ）银染法，对５０例原发性肺癌及自身正常肺组织３Ｐ１３２６上的５个微卫星位点进行检测。结果５０例肺癌中，微卫星不稳定发生率为５２％（２６／５０），杂合性丢失３８％（１９／５０）；低分化肺癌微卫星不稳定发生率（７７％）明显高于高、中分化（３６％）；微卫星不稳定联合杂合性丢失对肺癌的诊断阳性率达７２％。结论微卫星不稳定和杂合性丢失在肺癌发生、发展中可能起一定作用。

Objective To study the alteration of microsatellite DNA in primary lung cancer and its value of diagnosis for lung cancer. Methods Five microsatellite markers on chromosome 3P13 26 have been examined in 50 primary lung cancer tissues and their normal lung tissues. Results In fifty lung cancer,the incidence of microsatellite instability(MSI) was 52%(26/50) and the loss of heterozygosity (LOH) 38%(19/50). The positive rate of MSI in poorly differentiated lung cancer(13/17,77%)was significantly higher than that in well,moderately differentiated(10/28, 36%). The sensitivity in the diagnosis of lung cancer was 72% by combining MSI and LOH. Conclusions MSI and LOH may play a certain role in the carcinogenesis and progression of lung cancer and they may be valuable molecular markers in diagnosis of lung cancer.