p16、p53、p21基因对肺癌细胞增殖的影响

The efficacy of p16, p53 and p21 genes on the proliferation of lung cancer cells in vitro

目的观察肿瘤抑制基因ｐ１６、ｐ５３及细胞周期依赖激酶抑制物ｐ２１基因单独或联合应用时对肺癌细胞增殖的影响。方法应用十八酰基胺阳离子脂质体介导ｐ１６、ｐ５３、ｐ２１基因单独或共转染到非小细胞肺癌细胞系Ａ５４９和小细胞肺癌细胞系ＳＨ７７，观察转染后１、３、５日该细胞增殖的活力。采用四甲基偶氮唑盐微量酶反应比色法（ＭＴＴ法）测定吸光度（Ａ），以检测细胞增殖活力。结果Ａ５４９细胞系：Ａ均值分别为：空白组０．７８５６，脂质体组０．７４７８，ｐ１６组０．５５８９，ｐ２１组０．６０２２，ｐ５３组０．５７７８，ｐ１６＋ｐ２１组０．４７１１，ｐ１６＋ｐ５３组０．３０１７，实验组与对照组比较Ｐ＜０．０１，以共转染ｐ１６＋ｐ５３组最强。ＳＨ７７细胞系：Ａ均值分别为：空白组１．０２２２，脂质体组０．９３１６，ｐ１６组０．９１３７，ｐ２１组０．８８８９，ｐ５３组０．８３５６，ｐ１６＋ｐ２１组０．５９３３，ｐ１６＋ｐ５３组０．４２７８，单转染实验组与对照组比较Ｐ＞０．０５；共转染实验组与对照组比较Ｐ＜０．０１，以ｐ１６＋ｐ５３组更明显。结论ｐ１６、ｐ５３和ｐ２１基因有可能成为非小细胞肺癌基因替代治疗的候选基因，联合应用ｐ１６与ｐ２１基?

Objective Whether the p16, p21and p53 genes inhibit the proliferation of lung cancer cells after transfecting the genes alone or jointly was examined. Methods The p16 ,p21 and p53 genes mediated by Stearylamine/DOPE (SA liposome) were introduced alone and jointly into the non small cell lung cancer(NSCLC) cell line A549 and small cell lung cancer (SCLC) SH77.The effect of the genes on the growth of the cells was detected by MTT method at day 1,3,5 after gene transfection. Results The p16 , p53 and p21 genes alone mediated by SA liposome can inhibite obviously the growth of NSCLC cell line A549 cells, while the genes were slightly effective on SCLC cell line SH77 cells at day 1,3,5 after transfecting the genes. But co transfecting p16 and p21 or p16 and p53 can enhance the inhibiting effect on both A549 and SH77 cells significantly. Conclusions The tumor suppressor genes p16, p53 genes and CDK inhibitor p21 gene may be candidate for gene replacement therapy of NSCLC. Co transfecting p16 gene and p53 gene may be a better choice.