中药814对地鼠肺气肿防治作用的病理观察

The Morphometric Analysis of Lung on Protective Effect of Chinese Herbs 814 on Elastase Induced Emphysema in Hamsters

目的观察中药８１４对弹力酶诱导的金黄地鼠肺气肿的防治效果。方法随机设正常组、模型组、８１４防治组。模型组应用气管内注入弹力蛋白酶制造金黄地鼠肺气肿；防治组于注酶前两周以８１４灌胃。于实验开始后１月、２月、３月，分别测定三组动物的肺动脉压、血气、心脏指数、肺干湿质量比（ＤＷ／ＷＷ）；肺组织石蜡切片中ＭＬＩ、ＭＡＮ、ＰＡ／ＴＡ以显微微机图象处理系统测量。结果应用８１４后可使肺气肿组增加的肺干湿比恢复到与正常组无统计学差异；８１４防治组比单纯肺气肿组平均肺泡间隔（ＭＬＩ）减少，平均肺泡数（ＭＡＮ）、肺实质与总面积比（ＰＡ／ＴＡ）增加，皆有显著意义（Ｐ＜０．００１或Ｐ＜０．０５）。结论中药制剂８１４可减缓弹力酶引起的地鼠肺气肿病变的发生、发展。

Objective To determine the protective effect of Chinese herbal medicine 814 on elastase induced emphysema in hamsters. Methods Animals were injected intratrachealy with elastase for emphysematous models, prophylactic therapeutic groups were administrated with 814 through esophagus two weeks before the instillation of elastase untill animals were killed at three different time at first, second, and third month. Pulmonary artery pressure, blood gas analysis, heart index and the ratio of dried weight to wet weight of lungs (WW/DW) were examined. The lung paraffin sections were measured mean linear intercept (MLI), mean alveolar number (MAN), ratio of parenchyma area to total area (PA/TA) by the microscope computer morphometric analysis system. Results WW/DW in the prophylactic therapeutic groups was recovered at the same level with the controls, whereas the emphysematous were significantly increased (P<0.05); and compared with the emphysematous groups, the prophylactictherapeutic groups significantly decreased in MLI, increased in MAN and PA/TA (P<0.001 or P<0.05) Conclusions Administration of 814 could partly inhibit the development of emphysema induced by elestase in hamsters.