同步荧光法对三种芳香族氨基酸的测定

A Synchronous Spectrofluorimetric Method for the Determination of Three Aromatic Amino Acids in Food and Drug Injection

建立同时测定混合体系中色氨酸、酪氨酸和苯丙氨酸的同步荧光分析方法。以波长差Δλ=70nm进行同步荧光扫描,色氨酸、酪氨酸和苯丙氨酸的同步特征峰(以激发波长表示)分别位于280、228、206nm。酪氨酸和苯丙氨酸可直接由同步特征峰的信号强度进行定量;色氨酸的同步特征峰略受酪氨酸的弱峰影响,采用一阶导数处理后,即可消除干扰,实现定量分析;检出限分别为6.9×10-9、3.8×10-8、4.2×10-7mol/L。该方法无需预分离过程,用于啤酒及氨基酸注射液样品的测定,结果满意。

A synchronous spectrofluorimetric method is described for the determination of tryptophane, tyrosine and phenylalanine in food and drug injections in this paper. When λ was 70 nm for synchronous scanning, the characteristic peaks of tryptophane, tyrosine and phenylalanine appeared at 280, 228 nm and 206 nm, respectively. Tyrosine and phenylalanine could be directly quantified from their synchronous signals. The synchronous signal of tryptophane was slightly interfered by that of tyrosine, the interference from tyrosine could be effectively removed by the first order derivation synchronous fluorimetry. The detection limit was 6.9 ×10-9 mol/L for tryptophane, 3.8 ×10-8 mol/L for tyrosine and 4.2 × 10-7 mol/L for phenylalanine. No preliminary separation was needed for the determination by this method. It has been successfully applied to the analysis of beer samples and compound amino acids injection.