免疫印迹法对4种海虾主要过敏原的鉴定

Using Western Blotting to Identify Major Allergens of Four Species of Shrimp

以凡纳滨对虾、中国明对虾、刀额新对虾以及虾蛄4种海虾为研究对象,对这4种海虾过敏原蛋白组分进行研究。首先利用聚丙烯酰胺凝胶电泳对4种海虾过敏原组分进行分析,然后经免疫印迹鉴定其主要过敏原。结果显示:凡纳滨对虾的主要过敏原为99、33、19、14kD的蛋白质组分;中国明对虾的主要过敏原为39、33、28kD的蛋白质组分;刀额新对虾的主要过敏原为33kD和24kD的蛋白质组分;虾蛄的主要过敏原为56kD和48kD的蛋白组分。

Litopenaeus vannamei, Fenneropenaeus chinensis, Metapenaeus ensis and mantis shrimp were evaluated for their major allergens. Composition analysis of protein extracts from these four species of shrimp was carried out using SDS-PAGE, and western blotting was then used to identify their major allergens. The major allergens of Litopenaeus vannamei were protein fractions of 99, 33, 19 kD and 14 kD in molecular weight, and those of Fenneropenaeus chinensis protein fractions of 39, 33 kD and 28 kD, and those of Metapenaeus ensis protein fractions of 33 kD and 24 kD, and those of mantis shrimp protein fractions of 56 kD and 48 kD.