Preparation of Supermacroporous Composite Cryogel Embedded with SiO_2 Nanoparticles

Supermacroporous composite cryogels embedded with SiO2 nanoparticles were prepared by radical cryogenic copolymerization of the reactive monomer mixture of acrylamide（AAm） and N,N-methylene-bis-acrylamide（MBAAm） containing SiO2 nanoparticles（mass ratios of nanoparticles to the monomer AAm from 0.01 to 0.08） under the freezing-temperature variation condition in glass columns.The properties of these composite cryogels were measured.The height equivalent to theoretical plate（HETP） of the cryogel beds at different liquid flow rates was determined by residence time distribution（RTD） using tracer pulse-response method.The composite cryogel matrix embedded with the mass fraction of SiO2 nanoparticles of 0.02 presented the best properties and was employed in the following graft polymerization.Chromatographic process of lysozyme in the composite cryogel grafted with 2-acrylamido-2-methyl-1-propanesulfonic acid（AMPSA） was carried out to evaluate the protein breakthrough and elution characteristics.The chromatography can be carried out at relatively high superficial velocity,i.e.,15 cm·min-1,indicating the satisfactory mechanical strength due to the embedded nanoparticles.

与 SiO2 nanoparticles 嵌入的 Supermacroporous 合成 cryogels 被 acrylamide (AAm ) 和 N 的反应单体混合的激进的低温实验法的 copolymerization 准备，在玻璃列在结冰温度变化条件下面包含 SiO2 nanoparticles (到从 0.01 ～ 0.08 的单体 AAm 的 nanoparticles 的集体比率) 的 N-methylene-bis-acrylamide (MBAAm ) 。这些合成 cryogels 的性质被测量。到以不同液体流动率的 cryogel 床的理论板(HETP ) 的高度等价物被住处时间分发(RTD ) 用 tracer 脉搏反应方法决定。与 0.02 的 SiO2 nanoparticles 的集体部分嵌入的合成 cryogel 矩阵介绍了最好的性质并且在下列接枝聚合被采用。在有 2-acrylamido-2-methyl-1-propanesulfonic 酸(AMPSA ) 的合成 cryogel grafted 的溶解酵素的色析法的过程被执行评估蛋白质突破和 elution 特征。层析能在相对高的表面的速度被执行，即， 15 厘米 ? ‥髧躘? 用 ? 用吗？？