Eca109细胞光动力损伤效应的傅里叶变换红外光谱分析

Analysis of Fourier transform infrared spectrum for the photodynamic damage effect on Eca109 cell

目的:研究光敏剂血卟啉单甲醚(HMME)对人食管癌细胞Eca109的光动力损伤效应。方法:将Eca109细胞分为光动力组和对照组(不加光敏剂、不照光)。光动力组加入5mg/L的HMME培养6h后,用半导体激光器照射,光剂量为15J/cm2。采集对照组和光动力组细胞的傅里叶变换红外光谱,比较2组的图谱。结果:与对照组相比,光动力组蛋白质的α螺旋、β折叠含量减少,无规卷曲和β转角含量上升,磷酸二酯基团的对称伸缩振动1082cm-1和反对称伸缩振动1244cm-1谱带均向高波数移动,强度减小,CH2、CH3基团的对称和反对称伸缩振动的4条谱带峰值明显减弱。结论:光动力损伤作用使蛋白质主链的有序结构减少,无序性增加,磷酸二酯键损伤,DNA单、双链发生断裂,膜上多种功能基团结构受损,从而导致癌细胞凋亡或坏死。

Aim：To study the photodynamic damage effect on human esophageal cancer cell Eca109 by photosensitizer HMME.Methods：Eca109 cells were divided into photodynamic therapy（PDT） group and control group.Cells in PDT group were induced by HMME（5 mg/L） for 6 h,and then were exposed to laser at a dose of 15 J/cm2 using semiconductor laser as stimulating light source.The Fourier transform infrared spectrum（FTIR） of the two groups were collected and compared.Results：Compared with that of the control,the content of α helixes and β sheets for the protein in the PDT group decreased,and the content of random coils and β turns increased,the symmetry strain variation peak at 1 082 cm-1 and the asymmetric strain variation peak at 1 244 cm-1 of phosphodiester group both shifted to higher wave number,peak intensity decreased.Especially for the four spectral lines in the symmetry and asymmetric strain variation bands of CH2,CH3 function groups,peak intensity decreased noticeably.Conclusion：The photodynamic damage effect leads to protein main link＇s order structure decreasing and disorder structure increasing,phosphodiester bonds damaged,DNA single and double links broken,many structures of function groups on the thin membrane impaired,which results in the cancer cell apoptosis or necrosis.