1,25-二羟维生素D3对猪成釉细胞釉原蛋白和成釉蛋白分泌的影响

Effect of 1,25-(OH)_2 D_3 on the expression of amelogenin and ameloblastin secreted by porcine ameloblasts

目的:探讨1,25-二羟维生素D3[1,25-(OH)2D3]对体外培养的猪成釉细胞釉原蛋白(AMEL)及成釉蛋白(AMBN)分泌的影响。方法:原代培养猪成釉细胞,取第2代细胞12瓶,分为4组,每组3瓶。对照组不作加药处理,其余各组分别加入1×10-10、1×10-8及1×10-6mol/L的1,25-(OH)2D3,并分别于加药后1、3及5d取出1瓶,采用Western blot方法检测AMEL及AMBN的表达。结果:猪成釉细胞AMEL和AMBN的表达在不同浓度组间(F=359.688、619.718、117.412、859.364和49.099、705.946、338.824、119.352,P均<0.001)与不同作用时间组间(F=789.133、116.071、196.509和307.327、657.313、762.749,P均<0.001)差异均有统计学意义。其中AMEL的表达随作用时间延长和药物浓度的增加而增强,AMBN的表达随作用时间的延长增强,随药物浓度的增加而减少。结论:1,25-(OH)2D3可能通过调节AMEL及AMBN在釉质中的相对含量影响釉质的矿化过程。

Aim：To investigate the effect of 1,25-dihydroxyvitamin D3[1,25-（OH） 2D3]on amelogenin（AMEL） and ameloblastin（AMBN） secreted by cultured porcine ameloblast.Methods：A totle of 12 bottles of the second generation cultured porcine ameloblast were divided into four groups equally.Each bottle of the group were added 0,1 × 10-10,1 × 10-8,1 × 10-6 mol/L 1,25-（OH） 2D3,respectively.After 1,3 and 5 days treatment,the expression of AMEL and AMBN were detected using Western blot.Results：The expression of AMEL and AMBN in each 1,25-（OH） 2D3 concentration group and each time set were different,and there were obvious significance（F = 359.668,619.718,117.412,859.364,789.133,116.071,196.509,and 49.099,705.946,338.824,119.352,307.327,657.313,762.749,P 0.001）.The expression levels of AMEL increased with the time prolonged and the concentration of 1,25-（OH） 2D3 increased.The expression levels of AMBN increased with the time prolonged,while decreased with the concentration of 1,25-（OH） 2D3 increased.Conclusion：1,25-（OH） 2D3 may regulate the relative content of AMEL and AMBN in enamel,thus affect the enamel mineralization.