不同方法检测奶牛亚临床酮病的比较

Studies on the Different Detection of Subclinical Ketosis in Dairy Cows

本文以试剂盒测血清β-羟丁酸含量大于1.2mmol/L作为亚临床酮病奶牛的诊断标准,采用酮粉法、试剂法、ROSS法和试纸法分别对血酮、乳酮和尿酮含量进行检测,并将定性检测的结果与定量检测的结果相比较。结果表明,酮粉法测血酮的符合率最高、为88.54%;试纸法测尿酮次之、为87.50%;ROSS法测尿酮的符合率和阳性率最低,分别为79.17%和13.54%。酮粉法测血酮敏感性最高,为70.37%;ROSS法测尿酮的敏感性最低,为37.04%;试剂法测乳酮、ROSS法测尿酮和试纸法测尿酮三者的特异性均为95.65%,高于酮粉法测血酮的特异性(94.20%),酮粉法测尿酮特异性最低、仅为88.41%。从操作难易度和成本上考虑,酮粉法测定乳酮含量比较适合牧场亚临床酮病的检测。

In this study, serum β-hydroxybutyric acid （BHBA）eoncentration of 1.2mmol/L as the threshold for normal cows and subclinical ketosis cows, ketone powder, reagent, ROSS and test strip method to detect the blood, milk and urine ketones were applied respectively, and the results of qualitative with quantitative detection were compared. The results showed that blood powder test was the most accurate method, the coincidence rate was 88.54%, then urine ketones test with test strip, its rate of 87.50%; urine ketones test with ROSS was the method with the lowest coincidence and positive rates, the result were 79.17% and 13.54% respectively. Blood powder test has the highest sonsitivity（70.37%） and the lowest was urine test with ROSS（37.04%）; the specificity of reagent measuring milk ketone, ROSS and test strip method measuring urine ketones all were 95.65%, higher than that of blood powder method （94.20%）, the specificity of milk ketones test with ketone powder was lowest,only 88.41%. In consideration of difficulty of operation and cost, ketone powder detect milk is more suitable method on pasture detection of subclinical ketosis.