溃疡性结肠炎患者血浆和淋巴细胞内亲环素A测定的临床意义

Detection of Cyclophilin A in Plasma and Lymphocytes of Patients with Ulcerative Colitis

目的 探讨血浆和淋巴细胞内亲环素A（CyP A）水平与溃疡性结肠炎（ulcerative colitis,UC）的关系.方法 用酶联免疫吸附试验（ELISA）同时检测116例UC患者（其中活动期67例,缓解期49例）和50例正常人血浆和淋巴细胞内的CyP A水平.结果 活动期UC患者血浆和淋巴细胞内CyP A含量分别为（380±145）μg/L和（2 780±500）μg/g蛋白,均显著高于正常对照组的（160±78）μg/L （u值为10.54,P〈0.01）和（2 280±375）μg/g蛋白（u值为6.18,P〈0.01）,也显著高于缓解期UC患者（u值分别为8.19和5.37,P均〈0.01）.结论 血浆和淋巴细胞内CyP A升高可作为UC活动期诊断的辅助参考指标.

Objective To study the relationship between cyclophilin A （CyP A） and the activity of ulcerative colitis（UC）. Methods Plasma and lymphocytes levels of CyP A were detected by a double sandwich ELISA in 116 UC patients （67 active and 49 inactive UC patients） and 50 normal controls. The correlation between CyP A and the pathogenesis of UC. Resalts CyP A in plasma and lymphocytesof active UC patients was （380±145）μg/L and （2 780±500）μg/g protein, both statistically higher than normal control. Moreover,CyP A amount in plasma and lymphocytes of active UC patients was higher than that of inactive patients. Conclusion The increased CyP A amount in plasma and lymphocytes of UC patients is a useful marker for active UC.