胶乳凝集法用于细菌性脑膜炎检测的临床价值

Clinical Value of Diagnosis of Bacterial Meningitis by Latex Agglutination Test

目的 采用胶乳凝集法、革兰染色法及细菌培养法对脑脊液标本进行同步检测,探讨胶乳凝集法用于细菌性脑膜炎检测的临床价值.方法 对96例疑似细菌性脑膜炎患者采取脑脊液标本,分别进行革兰染色、胶乳凝集及细菌培养检查,采用SPSS11.0软件比较不同方法间的结果差异.结果 96例患者中直接革兰染色、胶乳凝集及细菌培养检查法分别检出病原菌30株、51株、8株.结论 胶乳凝集试验用于细菌性脑膜炎检测方便、快速,优于革兰染色法和细菌培养法,可作为细菌性脑膜炎的实验室辅助检测手段进行推广应用.

Objective Cerebrospinal fluid （CSF） specimens were collected for detecting pathogens by latex agglutination test（LPA）, Gram stain and culture method and evaluated Clinical value of diagnosis of bacterial meningitis by LPA test. Methods The study involved 96 cases of bacterial meningitis,LPA test,Gram stain and culture method were done in CSF for detection of pathogens with bacterial meningitis,compared the results between different methods. Results Of 96 specimens,using LPA test,an etiological diagnosis could be made 51 positive,in contrast,Gram stain and culture showed 30 and 8 positive,respectively. Conclusion LPA test was rapid,sensitive and superior to Gram stain and culture method, the study recommends LPA test as an adjuct test for routine diagnosis on bacterial meningitis.