血管内皮功能与2型糖尿病下肢血管病变的关系

The relationship between vascular endothelial function and lower limb angiopathy in type 2 diabetes mellitus

目的探讨2型糖尿病(T2DM)下肢血管病变(LLA)与血管内皮功能的关系。方法 71例T2DM患者通过彩色多普勒超声确诊的LLA严重程度分为:无LLA 26例、轻度17例、中度15例、重度13例;另选30名健康人为正常对照(NC)组。采用酶联免疫吸附双抗体夹心法,检测代表血管内皮功能的血浆血管性假血友病因子(vWF)和单核细胞趋化蛋白-1(MCP-1)水平。结果血浆MCP-1、vWF水平,糖尿病无LLA组明显高于NC组,有LLA组明显高于无LLA组,以及各LLA亚组间比较差异均有统计学意义(P均<0.01)。各LLA亚组下肢血管评分与血浆MCP-1(r=0.926,P<0.01)、vWF(r=0.942,P<0.01)水平正相关。结论血管内皮功能影响下肢血管病变。

Objective To investigate the relationship between vascular endothelial function and lower limb angiopathy（LLA） in type 2 diabetes mellitus. Methods 71 hospitalized T2DM patients were divided into four groups： 26 patients （group A） without LLA; 17 patients （group B） with light degree of LLA; 15 patients （group C） with middle LLA; 13 patients （group D） with critical LLA by color Doppler ultrasound. Normal control group were 30 subjects （NC group）. The plasma von Willebrand factor（vWF） and monocyte chemoattractant protein 1 （MCP-1） were determined by ELISA. Results The plasma MCP-1 and vWF level were higher in group A than in NC group, and higher in group B,C,and D than in group A（all P〈0. 01）. There were differences among groups B,C,D in MCP-1 and vWF （P〈0.01）. Scores of LLA were positively correlated with MCP 1 （r= 0. 926, P〈0. 01） and vWF（r= 0. 942, P〈 0. 01）. Conclusions The MCP-1 and vWF levels show a relationship with lower limb angiopathy in type 2 diabetes patients.