LM23基因沉默引起大鼠睾丸生精细胞凋亡

LM23-knockdown induced the apoptosis of spermatogenic cell in rat testis

目的研究LM23基因敲低后生精细胞的凋亡状况及与凋亡相关基因表达改变。方法用TUNEL方法检测睾丸细胞的凋亡情况,用大鼠全基因组表达谱芯片检测LM23基因敲低后凋亡相关基因的表达改变。结果 TUNEL结果显示,LM23基因敲低侧大鼠睾丸生精小管内大量生精细胞发生凋亡。大鼠全基因组表达谱芯片分析结果显示,许多促凋亡基因如Bcl-2家族的促凋亡基因表达上调,而抗凋亡基因如Faf1和Zfp91基因的表达明显下调。结论敲低大鼠的LM23基因,启动了Bcl-2家族介导的线粒体凋亡途径,引发了生精细胞发生大量凋亡。

Objective： To assess the apoptosis of spermatogenic cell and the expressions of genes related to apoptosis in LM23-knockdown rats. Methods： TUNEL assay was used to assess the apoptosis of spermatogenic cell. The expressions of genes related to apoptosis were surveyed by microarray analysis using Agilent rat whole genome arrays in LM23-knockdown rat testis. Results： A TUNEL assay showed the presence of many apoptotic cells in seminiferous tubules. Mi- eroarray analysis found that many pro-apoptotic genes were up-regulated such as Bcl-2 family members ineluding Bax, Bid3 and Bakl. And many anti-apoptotic genes were down regulated such as Fafl and Zfp9. Conclusions： LM23-knockdown started up Bcl-2 family mediated mitochondrial-apoptosis pathway, consequently induced the apoptosis of spermatogonial cells in rat testis.