PBS/PLA共混材料的制备及其细胞相容性研究

Cytocompatibility of PBS/PLA blend as the sternal fixation material

目的本论文对聚丁二酸丁二醇酯/聚乳酸(PBS/PLA)合金作为胸骨固定材料的细胞相容性和细胞毒性进行了深入地研究。方法首先采用CCK-8试剂盒表征PBS/PLA合金浸提液对L929细胞生长增殖的影响;其次将细胞直接种于PBS/PLA材料的表面评价该合金与L929细胞的相容性情况;最后利用PE,PVC作为对照材料,评价合金对细胞的毒性级别。结果和结论 PBS/PLA合金对于L929细胞具有良好的细胞相容性且无明显细胞毒性,在其表面的繁殖塑料甚至优于医用聚乙烯材料。证明PBS/PLA合金能够作为胸骨捆扎固定材料。

To evaluate biocompatibility and cytotoxicity of PBS/PLA blend as the material for sternal fixation. Methods L929 cell line was used to assess the cytotoxicity and cytocompatibility of PBS/PLA blend by incubation of the cells with the aqueous extract of the material for 3 days. The cytocompatibility of the material was evaluated by observing the proliferation of the cells cultured on the surface of the material. The proliferation of the cells cultured on the surface of the material was compared with that of the cells cultured on medical polyethylene and polyvinyl chloride to assess the cytotoxcity of the material. Results L929 cells showed no morphological changes after a 3-day incubation with the aqueous extract of the material. The cells cultured on the surface of the blend material exhibited better proliferative activity than those cultured on medical polyethylene and polyvinyl chloride, demonstrating no obvious cytotoxicity of the material. Conclusion PBS/PLA blend material possesses excellent cytocompatibility and can be a promising biodegradable polymer for sternal fixation.