子宫内膜炎患牛子宫内膜组胺浓度与MC颗粒状态的研究

The Study on Concentration of Endometrial Histamine and Mast Cell Graininess in Endometritis Cow

本研究旨在探讨子宫内膜肥大细胞(MC)颗粒状态和组胺浓度在奶牛子宫内膜炎中的作用。选用产后6～10 d健康及患急性化脓性子宫内膜炎的中国荷斯坦奶牛各10头,分别为对照组和试验组,通过ELISA法检测子宫内膜组胺浓度和透射电镜观察子宫内膜MC颗粒状态。结果表明:试验组子宫内膜组织中组胺含量为(80.305±4.002)ng.mL-1,对照组为(39.204±4.278)ng.mL-1,二者差异极显著(P<0.01);对照组子宫内膜固有层MC胞质中可见大小不等,圆形或椭圆形颗粒,均匀分布;而试验组子宫内膜固有层MC呈脱颗粒状态,表现为颗粒数量减少、分布和浓度不均、周腔增大、有空泡形成。结果提示,产后发生急性子宫内膜炎时子宫内膜MC脱颗粒,释放组胺,使子宫内膜组胺含量显著升高,这与子宫内膜的急性炎症有关,组胺可调节子宫免疫机能,可能对子宫内膜炎的发生发展具有重要作用。

The study was focused on investigating the role of endometrial mast cells（MC） grains particle state and the histamine concentration in cow endometritis.Healthy and suffering from acute purulent endometritis Chinese Holstein cow were selected as control group and experimental group respectively,the number of each group was 10 and 6-10 days post-natal.The endometrial histamine concentration was detected by ELISA and the endometrial MC particle state was checked by transmission electron microscopy.The results showed that the concentration of endometrial histamine was（80.305±4.002） ng·mL-1,（39.204±4.278） ng·mL-1 in experimental group and in control group,the difference was more significant（P〈0.01）.In control group,inequality of size,homogeneous distribution,round/oval granule were discovered in MC cytoplasm of endometrium lamina propria;In experimental group,MC was degranulated,the quantity of granule decreased,the distribution was unevenness,cavity increment and vacuolization.Conclusion indicated that MC degranulation,released histamine in postpartum acute endometritis,leaded to the content of endometrial histamine significant increase,it concerned with endometrial acute inflammation,histamine could regulate the immunity function of uterus.It was very important to development of endometritis.