溴代呋喃酮对胸心外科聚氯乙烯材料表面表皮葡萄球菌生物膜形成的影响

The Effect of Brominated Furanones on the Biofilm Formation of Staphylococcus Epidermidis on the Polyvinyl Chloride Material

目的探讨溴代呋喃酮对胸心外科聚氯乙烯(PVC)材料表面表皮葡萄球菌生物膜形成的影响,为生物材料表面改性研究及临床生物材料植入感染的防治提供新思路。方法选用化学结构具有代表性的三种溴代呋喃酮分为3组,呋喃酮1组:3,4-二溴基-5-羟基-呋喃酮;呋喃酮2组:4-溴-5-(4-甲氧基苯基)-3-(甲氨基)-呋喃酮;呋喃酮3组:3,4-二溴基-5,5-二甲苯基-2(5H)-呋喃酮;对照组:PVC材料用酒精浸泡5min;分别对4组PVC材料进行表面涂层改性,将改性过的PVC材料与表皮葡萄球菌共同培育;分别于培养6h、12h、18h和24h时用激光共聚焦显微镜动态观察PVC材料表面细菌群落及细菌生物膜厚度的形成,扫描电子显微镜观察PVC材料表面细菌生物膜表面结构。结果激光共聚焦显微镜观测结果显示:呋喃酮2组各时间点PVC材料表面表皮葡萄球菌群落数量和细菌生物膜厚度明显小于对照组(P<0.05),呋喃酮1组、呋喃酮3组表皮葡萄球菌群落数量和细菌生物膜厚度与对照组比较差异无统计学意义(P>0.05)。扫描电子显微镜观察显示:与对照组比较,呋喃酮2组6h时PVC材料表面细菌群落附着数量较少;18h时对照组PVC材料表面细菌生物膜结构初步形成,而呋喃酮2组无明显细菌生物膜结构形成。结论不同溴代呋喃酮对PVC材料表面表皮葡萄球菌生物膜形成的影响不同,呋喃酮2可以抑制PVC材料表面表皮葡萄球菌群落数量和细菌生物膜厚度的形成。

Objective To study the influence of brominated furanones on the biofilm （BF） formation of Staphylococcus epidermidis （SE） on polyvinyl chloride（PVC） materials,and provide new ideas for the research of surface modification of materials and clinical treatment of biomaterial centered infection.Methods We chose three kinds of brominated furanone with representative chemical structure for our research which were respectively 3,4-dibromo-5-hydroxy-2 （5H）-furanone （Mucobromic acid） in the first furanone group,4-bromo-5-（4-methoxyphenyl）-3-（methylamino）-2（5H）-furanone in the second furanone group,and 3,4-dibromo-5,5-bis（4-methylphenyl）-2（5H）-furanone in the third furanone group.The PVC material soaked with 75% ethanol for 5minutes was classified as the control group.The surface coating of the PVC materials in the four groups all underwent modification respectively and then they were co-cultivated with staphylococcus epidermidis together.Confocal laser scanning microscope（CLSM） was adopted to detect the thickness of bacterium BF and bacterium community quantity unit area on PVC materials and scanning electron microscope（SEM） was used to observe surface structure of SE,BF formation at 6 h,12 h,18 h and 24 h respectively.Results The results of CLSM showed that,compared with the control group,SE bacterium community quantity unit area and the thickness of bacterium BF on the PVC material surface in the second furanone group were obviously smaller （P〈0.05）.SE bacterium community quantity unit area and thickness of bacterium BF on PVC material surface in the first and the third furanone groups had no significant difference （P〉0.05）.The result of SEM showed that,the quantity of SE bacterium community unit area on PVC material surface in the second furanone group were smaller than that of the control group at 6 hours.The biofilm structure on PVC material surface in the control group was formed at 18 hours,but there were no mature biofilm structure on PVC material surface in the second furanone group at 18 hours.Conclusion The impact of different brominated furanone on SE biofilm formation on the surface of PVC materials is different.The second kind of furanone can inhibit the quantity of SE bacterium community unit area and SE biofilm formation on the surface of PVC materials.