摘要
研究ABCE1对肺癌(95-D和NCI-H446)细胞的作用.使用RNA干扰技术,抑制ABCE1基因的表达,通过Western blot分析及FACS检测,观察ABCE1基因对E-钙黏附蛋白在95-D/NCI-H446细胞表达的影响;运用transwell侵袭实验,观察M95-D/NCI-H446细胞侵袭力的变化.RNA干扰ABCE1基因后,实验组与对照组相比,在48h后可显著抑制肺癌(95-D和NCI-H446)细胞ABCE1蛋白的表达,同时,伴随E-钙黏附蛋白的高表达,以及细胞侵袭力的降低.ABCE1基因与E-钙黏附蛋白相关,抑制ABCE1基因可增加肺癌95-D/NCI-H446细胞的E-钙黏附蛋白的表达,减低细胞的侵袭力。
To investigate further the role of ABCE1 in 95-D/NCI-H446 lung carcinoma cells,the protein of ABCE1 was knocked-down using an RNAi approach.Effect of siRNA-expressing vector on ABCE1 and E-cadherin protein expression was evaluated in 95-D/NCI-H446 lung carcinoma cells by Western blot and FACS analysis.A transwell collagen invasion assay was used to assess differences in the invasive properties of cells expressing ABCE1.Treatment of the 95-D or NCI-H446 cells with an RNAi to ABCE1 led to near 85% knockdown of ABCE1 expression after 48 h.Interestingly,knockdown of ABCE1 expression was accompanied by a significantly higher level in E-cadherin expression.And the expression of E-cadherin in siRNA-95D/H446 was much higher than that in either of the control cell lines by FACS analysis.Moreover,cells treated with the ABCE1 RNAi displayed poor less invasion than the control cell lines too.There were close relationships between ABCE1 and E-cadherin.It has been suggested that silencing ABCE1 expression increases E-cadherin expression and decreases cell invasion in 95-D /NCI-H446 lung carcinoma cells.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2010年第8期891-896,共6页
Progress In Biochemistry and Biophysics
基金
supported by grants from Natural Science Foundation of Hebei Province(C2009001262)
Research Special Foundation of Health of Hebei(08176)~~