血管内皮生长因子在宫腔粘连患者子宫内膜中的表达

Expression of endometrial VEGF in patients with intrauterine adhesion

目的通过检测宫腔粘连(IUA)患者子宫内膜血管内皮生长因子(VEGF)的表达,探讨VEGF在宫腔粘连发病中的作用。方法选择经宫腔镜诊断为宫腔粘连的患者35例为IUA组,因男性不孕在本院行人工受精治疗的女性25例为对照组,采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶连结法(SP)检测两组患者子宫内膜组织中VEGF的表达水平。结果粘连组子宫内膜VEGF表达水平为(3.53±0.16)分,对照组为(3.22±0.08)分,粘连组高于对照组;宫腔粘连患者VEGF表达水平重度为(3.87±0.25)分、中度为(2.45±0.10)分、轻度为(2.28±0.10)分,重度宫腔粘连患者VEGF表达水平明显高于轻、中度,差异均有统计学意义(P<0.01)。结论子宫内膜VEGF的异常表达,可能参与宫腔粘连的形成。

Objective To elucidate the possible mechanism of intrauterine adhesion （IUA） by detecting the expression of VEGF in endometrium. Methods A total of 35 patients who were diagnosed as IUA by hysteroscopy were included in the study group, and 25 women who receive in vitro fertilization treatment due to male factor were used as the control group. Immunohistochemieal method was performed to detect the expression of endometrial VEGF in the two groups. Results The expression of endometrial VEGF increased significantly in the study group compared with the control group （P 〈 0. 01 ）. In the study group, the expression of endometfial VEGF of those with serious IUA was extremely higher than those with mild and moderate IUA （ P 〈 0. 01 ）. Conclusion The abnormal expression of VEGF may play a role in the occurrenee of IUA.