垂体生长激素腺瘤中gsp癌基因对GHSR-1a、Ghrelin mRNA表达的影响

Effect of gsp oncogene on expression of GHSR - la and Ghrelin mRNA in human GH - secreting pituitary adenomas

目的 研究垂体生长激素（GH）腺瘤组织中Ghrelin、生长激素释放剂受体-1a亚型（GHSR-1a）mRNA的表达,并探讨其表达与gsp癌基因的关系.方法 采用PCR-DNA直接序列分析方法,观察43例垂体GH腺瘤组织中gsp癌基因的表达;应用实时荧光定量PCR检测Ghrelin和GHSR-1a mRNA的表达水平;统计学分析Ghrelin和GHSR-1a mRNA表达与gsp癌基因的关系.结果 Ghrelin mRNA表达在gsp癌基因阳性、阴性组织间差异无统计学意义（P＞0.05）;gsp阳性组织中GHSR-1a mRNA表达明显高于gsp阴性组织（P＜0.05）;gsp阳性、阴性腺瘤组织中GHSR-1amRNA与Ghrelin mRNA的表达均呈明显正相关（R=0.592或0.544,P＜0.05）.结论 垂体GH腺瘤中gsp癌基因上调GHSR-1a mRNA的表达;而对Ghrelin mRNA表达无明显影响.gsp阳性、阴性腺瘤中Ghrelin均可正向调控GHSR-1a mRNA表达.

Objective To investigate the relationship between the expression of growth hormone secretagogue receptor type 1a （GHSR- 1a） 、Ghrelin mRNA and gsp oncogene in human GH- secreting pituitary adenomas. Methods The gsp oncogene mutation in 43 cases of GH -secreting pituitary adenomas was detected by PCR - DNA direct sequencing analysis. The expression of GHSR - 1a、Ghrelin mRNA was determined by SYBR green real -time fluorescent quantitative PCR. Then the relationships between the expression of GHSR -1a、Ghrelin mRNA and gsp oncegene were studied with statistical methods. Results There was no significant difference in the expression of ghrelin mRNA between mutation - positive and mutation - negative adenomas. The expression of GHSR - 1a mRNA was significantly higher in gsp - positive adenomas than that in gsp - negative adenomas. There was significant direct correlation between the levels of ghrelin mRNA and GHSR mRNA expression in gsp - positive and gsp - negative adenomas. Conclusions Gsp oncogene may up - regulate the expression of GHSR - 1a mRNA and has no effect on the expression of ghrelin mRNA in human GH - secreting pituitary adenomas. In addition, ghrelin could elevate the expression of GHSR - 1a mRNA in GH - secreting pituitary adenomas with or without gsp mutations.