非小细胞肺癌患者外周血CEA mRNA表达及临床意义

Expression and Clinical Significance of CEA mRNA in Peripheral Blood of Non-small Cell Lung Cancer Patients

目的检测非小细胞肺癌(NSCLC)患者外周血CEA表达情况,探讨其作为分子标志物检测NSCLC微转移的可行性。方法应用RT-PCR技术检测48例NSCLC患者,15例肺良性疾病(BLD)患者和10例健康人外周血CEAmRNA的表达。结果 NSCLC患者CEAmRNA的阳性表达率明显高于BLD患者和健康对照组(P<0.01)。Ⅰ～Ⅳ期NSCLC患者外周血中CEAmRNA阳性表达率分别为27.8%、83.3%、61.5%和100%。NSCLC患者外周血CEA阳性表达与临床分期及淋巴结转移密切相关(P<0.05),而与病理类型及分化程度无关(P>0.05)。结论检测外周血CEAmRNA表达可作为诊断NSCLC患者微转移的一个有价值的参考指标。

Objective To detect CEA expression in peripheral blood of non-small lung cancer（NSCLC） patients and discuss the possibility of indicating NSCLC micrometastases by it.Methods The expression of CEA mRNA was analyzed by RT-PCR in 48 NSCLC in peripheral blood,compared with 15 patients with benign lung disease（BLD）and 10 healthy volunteers.Results The positive expression rate of CEA mRNA was significantly higher in NSCLC than in those of BLD and healthy volunteers（P0.01）.The expression rate of CEA mRNA was 27.8%,83.3%,61.5%,100% in peripheral blood with non-small cell lung cancer of TNM stageⅠ～Ⅳrespectively.The positive expression of CEA was closely related with the clinical staging and lymph node metastasis（P0.05）,but not related to pathological type and tumor cell differentiation（P0.05）.Conclusion Expressions of CEA mRNA in peripheral blood can be used as a valuable biomarker for diagnosis of micrometastasis in patients with NSCLC.