抗纤软肝颗粒对肝纤维化大鼠肝组织核因子-κB基因表达的影响

The effect of Kangxian ruangan granule on the expression of nuclear factor-κB gene in fibrotic rat liver

目的观察中药抗纤软肝颗粒对四氯化碳加乙醇诱导肝纤维化大鼠的肝组织核因子-κB(NF-κB)p65 mRNA表达的影响。方法 SD雄性大鼠40只,随机分为模型对照组(M)15只,抗纤软肝颗粒组(K)15只,正常对照组(N)10只。M组、K组分别以40%四氯化碳橄榄油液腹腔注射,每周2次,并以5%乙醇为唯一饮水。造模同时,K组即予抗纤软肝颗粒20 g.kg-1.d-1(中等剂量)灌胃,每天一次。7周末,各组取肝右叶分别作肝组织病理观察及检测肝组织NF-κB p65 mRNA表达。结果抗纤软肝颗粒组肝细胞变性坏死及肝纤维化水平较模型对照组明显减轻(P<0.05),肝组织NF-κB p65 mRNA表达较模型对照组明显减弱(P<0.05)。结论抗纤软肝颗粒能下调肝纤维化大鼠肝组织NF-κB p65 mRNA表达,抑制肝纤维化的形成和发展,其机制可能是通过调控NF-κB p65 mRNA转录过程实现的。

Objective To study the effect of Kangxian Ruangan granule on the expression of nuclear factor-κB（NF-κB） p65mRNA in rats with hepatic fibrosis induced by ethanol and CCl4.Methods 40 male SD rats were randomly divided into 3 groups： Model control group（M,n=15）,Kangxian Ruangan granule group（K,n=15） and normal control group（N,n=10）.In groups M and K,the rat hepatic fibrosis model was induced by intraperitoneal injection of 40% CCl4 in olive oil twice a week and 5% ethanol as the only drinking water.The rats of K Group were given 20g/kg/day body weight（medium dose） of Kangxian Ruangan granule through stomach tubes.At the end of the 7th week,the structural changes of liver tissue and the expression of NF-κB p65 were examined by liver tissue biopsy and in situ hybridization techniques,respectively.Results The degenerative necrosis and liver fibrosis were improved significantly in K group compared to the M group（P0.05）.The expression of NF-κB p65 decreased dramatically in M group（P0.05）.Conclusion Kangxian Ruangan granule down regulates the expression of NF-κBp65 in rat liver tissue and inhibits the formation and development of liver fibrosis.The mechanism of anti-liver fibrosis of Kangxian Ruangan granule may be achieved by regulating the transcription process of NF-κBp65.