胰腺导管腺癌组织中PUMA、P53、Bax和Bcl-2蛋白的表达及临床意义

Expression of PUMA,P53,Bax and Bcl-2 proteins in pancreatic ductal adenocarcinoma and the relevant clinical significance

目的观察胰腺导管腺癌(pancreatic ductal adenocarcinoma,PDA)组织中PUMA、P53、Bax和Bcl-2蛋白的表达,探讨PUMA在PDA发生、发展中的作用及其与P53、Bax和Bcl-2蛋白表达的关系。方法采用免疫组织化学EnVision方法检测65例PDA组织及配对癌旁组织中PUMA、P53、Bax和Bcl-2蛋白的表达情况。结果 PDA中PUMA蛋白阳性表达率为30.8%(20/65),明显低于相应癌旁组织49.2%(32/65),差异有统计学意义(P<0.05)。不同肿瘤大小及有、无淋巴结转移患者肿瘤组织PUMA表达差异有统计学意义(P<0.05),而不同年龄、性别、肿瘤部位、分化程度、肿瘤分期和有无神经浸润患者PUMA表达差异无统计学意义。肿瘤组织PUMA蛋白表达与P53、Bcl-2蛋白表达均负相关(P=0.019,P=0.015),而与Bax蛋白表达无相关性。结论 PDA组织中PUMA蛋白表达下调与肿瘤大小、淋巴结转移有关,PUMA可能参与了PDA的发生、发展,其蛋白表达水平的检测可能有利于PDA预后判定。

Objective To observe the expression of PUMA,P53,Bax and Bcl-2 protein in pancreatic ductal adenocarcinoma（PDA）,and to investigate the role of PUMA in tumorigenesis of PDA and its relationship with P53,Bax and Bcl-2 protein expression.Methods The expression of PUMA,P53,Bax and Bcl-2 protein was examined by immunohistochemical EnVision method in 65 PDA tissues and the corresponding adjacent tissues.Results The positive rate of PUMA protein expression was 30.8%（20/65） in the PDA tissues,which was significantly lower than that in the corresponding adjacent tissues（49.2% [32/65],P.05）.The expression of PUMA protein was significantly correlated with the tumor size and lymph node metastasis（P0.05）,but not with patient＇s age,sex,tumor location,differentiation degree,tumor stage or neural invasion.PUMA expression was negatively correlated with P53 and Bcl-2 expression（P=0.019,P=0.015）,but was not correlated with Bax expression in PDA tissues.Conclusion Decreased PUMA protein expression is correlated with tumor size and lymph node metastasis in PDA patients,suggesting that PUMA protein might be involved in the tumorigenesis and development of PDA.Detection of PUMA protein expression may be used for predicting the prognoses of PDA patients.