南欧派利吞草石油醚提取物对雄性大鼠性行为的影响(英文)

Effects of petroleum ether extract of Anacyclus pyrethrum DC. on sexual behavior in male rats

目的:检测南欧派利吞草石油醚提取物(petroleum ether extract,PEE)对雄性大鼠性行为的影响。方法:制备南欧派利吞草PEE。32只雄性Wistar大鼠分成对照组、睾酮组、低剂量PEE组和高剂量PEE组,每组8只。对照组大鼠灌胃花生油(溶剂对照),睾酮组大鼠肌肉注射0.5mg/kg睾酮,每周2次,低剂量和高剂量PEE组大鼠分别灌胃50和100mg/kgPEE,治疗持续28d。于治疗前,治疗第15天、第28天和治疗后第7天、第15天,检测大鼠体质量,治疗结束后,取4只大鼠性器官(睾丸、附睾、精囊、前列腺)称取质量,其余4只大鼠用于检测大鼠的性取向行为和性功能。结果:与对照组大鼠比较,PEE治疗28d可明显增加雄性大鼠的体质量和性器官质量,雄性大鼠易于接受雌性大鼠和对雌性大鼠趋向性增加,雄性大鼠性能力增加,表现为舔、嗅雌性大鼠肛门和生殖器,阴茎勃起指数增加,交配潜伏期缩短,交配频率增加。在PEE治疗结束后7和15d仍观察到以上行为和性指标的变化。结论:与睾酮不同的是,PEE治疗结束后7和15d仍可见明显治疗效果,提示南欧派利吞草能提高大鼠性潜能,并有延迟效应。

Objective：The roots of Anacyclus pyrethrum DC.（Compositae） are employed in Ayurvedic system of medicine as ＂Vajikaran Rasayana＂ — a category of drugs for vitality and virility.They are believed to have aphrodisiac action.The present investigation was undertaken to evaluate their effects on sexual behavior in male rats.Methods：Thirty-two male Wistar rats were divided into control group,testosterone group,low-dose （50 mg/kg） petroleum ether extract （PEE） group and high-dose （100 mg/kg） PEE group.PEE obtained from the roots of Anacyclus pyrethrum was administered orally to albino rats once daily,and 0.5 mg/kg （body weight） of testosterone was given intramuscularly twice weekly and served as positive control.The course of treatment was 28 days.The effects of PEE and testosterone on changes in body and accessory sexual organ weights,sexual behavior,penile erection and sexual performance were studied before treatment,after 15 and 28 days of treatment and 7 and 15 days after treatment.Results：After 28 days of treatment,PEE and testosterone had a marked influence on body and accessory sexual organ weights as compared with arachis oil.The treated male rats were more receptive and oriented towards female rats and increased precopulatory activities like licking and sniffing of female anogenitals were observed.The penile erection index was significantly increased with reduction in mount latency and intromission latency period.There were four-fold increase in mount and three-fold increase in intromission frequency in treated rats reflecting improved sexual performance.The behavioral and sexual parameters were also observed after a lapse of 7 and 15 days of discontinuance of drug treatment.Conclusion：Unlike testosterone,the PEE of Anacyclus pyrethrum shows efficacy in rats tested after the lapse of 7 and 15 days of discontinuation of treatment.This suggests that the drug has prolonged effect and capacitate the treated rats for improved sexual potential.