气相色谱法同时检测茶叶中12种农药残留

Determination of 12 pesticides residues in tea by gas chromatography

目的:建立同时检测茶叶中12种有机磷和有机氯农药残留的气相色谱法。方法:用丙酮和石油醚混合溶剂浸提,石墨化碳柱固相萃取。采用电子捕获检测器、程序升温、OV-1701(30 m×0.25 mm×0.25μm)毛细管柱气相色谱法检测。结果:12种农药的回收率为79.0%～102.1%,相对标准偏差RSD为1.87%～3.34%;检出限为0.008 mg/kg～0.051 mg/kg;12种农药线性范围为0.1 mg/L～15.0 mg/L,相关系数r≥0.9923。结论:该方法简便、灵敏、准确,适用于分析茶叶中的12种有机磷和有机氯农药残留含量。

Objective：To establish gas chromatographic method for the determination of organochlorine and organophosphorous pesticide residues in tea.Methods：The analytes were extracted with acetone and petroleum ether,purified with graphite carbon column and detected by ECD.Results：The average recoveries and relative standard deviations（RSDs）for the analysis were in the range of 79.0%～102.1% and 1.87%～3.34%%（n=5）,respectively.The limits of detection were 0.008 mg/L～0.051 mg/L.Good linearities were obtained in the concentration ranging from 0.1 mg/L～15.0 mg/L,the correlation coefficients were higher than 0.9923.Conclusion：The method is simple,rapid,sensitive which can meet the needs for organochlorine and organophosphorous pesticide residues in tea.