脉冲场凝胶电泳分型应用于奇异变形杆菌食源性疾病的溯源

Application of pulse-field gel electrophoresis analysis (PFGE) in the source-tracking of food-borne disease caused by Proteus mirabilis

目的:应用脉冲场凝胶电泳(PFGE)技术对奇异变形杆菌食源性疾病进行分析。方法:采用限制性内切酶Sfi I,对广州市二起疑似奇异变形杆菌食源性疾病中22株奇异变形杆菌进行PFGE分子分型,用BioNumerics Version 4.0软件(复选Dice相关系数和UPGMA方法)进行聚类分析。结果:以80%相似度为标准,22株奇异变形杆菌被分成7个PFGE克隆型。同起食源性疾病菌株克隆型相似度较高,不同起食源性疾病菌株克隆型有交叉但存在较大差异。结论:PFGE分子分型与流行病学资料紧密结合可增强对变形杆菌食源性疾病的溯源和预警。

Objective：To apply pulse-field gel electrophoresis analysis（PFGE） in the analysis of a case of food-borne disease caused by Proteus mirabilis.Methods： PFGE using restriction enzyme Sfi I was employed in the molecular subtyping of Tweenty-two strains of P.mirabilis isolated in a case of food poisoning in Guangzhou city and PFGE patterns were analyzed by BioNumerics Version 4.0 software to perform cluster analysis.Pattern profiles were compared by utilizing the Dice coefficient and UPGMA（unweighted pair group method with arithmetic averages）.Results： Seven different PFGE patterns were observed in the 22 P.mirabilis strains,with similarity 80% as criteria,strains with same food-borne disease showed a high pulsotype similarity,strains from different food-borne disease produced cross-pulsotype but significant difference.Conclusion： It is evident that PFGE subtyping,coupled with epidemiological data,which can enhance source-tracking of and warning against food-borne disease caused by P.mirabilis.