RET与VEGF—C在甲状腺乳头状癌中表达的研究

Study on expression of RET and VEGF - C in papillary carcinoma of thyroid

目的探讨RET和VEGF—C在甲状腺乳头状癌发生发展中的作用，以及RET和VEGF—C之间相互作用的关系。方法应用免疫组化二步法检测60例甲状腺乳头状癌和10例正常甲状腺组织中RET蛋白和VEGF—C蛋白的表达情况，分析RET和VEGF—C的蛋白表达率与临床病理学特征之间的关系以及二者表达之间的相关性。结果RET和VEGF—C在甲状腺乳头状癌与甲状腺正常组织中的阳性表达率之间均具有显著性差异（P〈0．05）。RET和VEGF—C与患者的性别、年龄、肿瘤大小无显著差异（P〉0．05）。RET和VEGF—C与患者的颈部淋巴结转移、临床分期有显著差异（P〈0．05）。RET和VEGF—C之间呈正相关（P〈0．05）。结论RET表达在甲状腺乳头状癌淋巴结转移方面具有非常重要作用，其有可能通过调节VEGF—C的表达来促进淋巴结的转移。RET和VEGF—C可作为判断甲状腺乳头状癌淋巴结转移的可能指标。

Objective To investigate the expression of RET and VEGF - C in papillary carcinoma of thyroid, Soasto the role and correlation of RET and VEGF - C. Methods Detecting the expression of RET and VEGF - C in 60 papillary carcinoma of thyroid tissues and 10 normal thyroid tissues by immunohistoche mical Envision two - step method, analyzing the correlation between the protein expression rate and clinical and pathological characters,and also the correlation between the expression of RET and VEGF - C. Results Expression of every oncogene was significantly different between papillary carcinoma of thyroid tissues and normal thyroid tis- sues（ P 〈0.05 ）. The positive expression rate of RET and VEGF- C were significantly associated with cervical lymph node metastasis and clinical stages（ P 〈0.05 ） , but not associated with sex, age, primary site of the tumor （ P 〉 0.05 ）. The positive expression of RET closely related to the positive expression of VEGF - C （ P 〈 0.05 ）. Conclusion The results demonstrate that RET may play an important role in the cervical lymph node metastasis of papillary carcinoma of thyroid,the expression of VEGF - C. RET and VEGF - C should be used as an important marker of cervical lymph node metastasis.