超临界CO_2萃取锁阳中黄酮的工艺研究

Study on Supercritical CO_2 Extraction of Flavonoids from Cynomorium songaricum

目的:探索用超临界CO2萃取锁阳中黄酮类化合物的最佳工艺条件。方法:采用单因素实验和正交实验研究了压力、温度、时间、夹带剂浓度、夹带比(夹带剂流量与CO2流量的百分比)、CO2流量、锁阳颗粒度(过筛目数)等对锁阳中黄酮萃取率的影响,确定了最佳工艺条件。结果:超临界CO2萃取锁阳中黄酮的最佳条件为:锁阳颗粒60～80目,压力30MPa,温度50℃,时间75min,夹带剂浓度50%,夹带比8%,CO2流量5mL/min;萃取率为21.18%。结论:该工艺具有萃取率高、速度快、操作简便等优点,可用于锁阳中黄酮类化合物的提取。

Objective ：To study the extraction technology of flavonoids from Cynomorium songaricum by supercritical CO2 extraction. Methods ：The effects of pressure,temperature,time,concentration of alcohol,dosage of chemical preparation,flux of CO2 and particle size were studied by single factor analysis and orthogonal test. Results ：The optimized conditions were as follows：particle size 60~80 sieve mesh,the pressure was 30 MPa,the temperature was 50 ℃,the time was 75 min,concentration of alcohol was 50%,entrainment rate was 8%,flux of CO2 was 5 mL/min. The total flavonoids yield could reach 21.18% under the above conditions. Conclusion ：This method is simple,rapid and higher extraction yield,so it is suitable for the extraction of flavonoids from Cynomorium songaricum.