摘要
采用Plackett-Burman试验设计及响应面分析法,对一株白色链霉菌发酵ε-聚赖氨酸培养基进行优化。首先利用Plackett-Burman试验设计筛选出显著影响产ε-聚赖氨酸的因素,再利用最陡爬坡路径逼近最大响应区域,最后在此基础上利用中心组合试验及响应面回归分析确定最优培养基。结果表明,葡萄糖、(NH4)2SO4与ε-聚赖氨酸产量存在显著的相关性,其最适浓度分别为33.196,8.572g/L,在优化条件下,ε-聚赖氨酸产量达到(2.491±0.124)g/L与预测值2.543139g/L非常接近,产量提高了63.6%。
Plackett-Burman design and response surface method were applied to obtain optimum media forε-Polylysine production by streptomyces albus KD-11.Firstly,Plackett-Burman design was used to evaluate the remarkable variables which were relevant to the production of ε-Polylysine.Then the greatest response area was studied by steepest ascent approach design.Finally,the optimum medium was selected using the central composite design and Response Surface Methodology.The results showed that both ammonium sulfate and glucose have significant influence onε-Polylysine yield which optimum concentrations were 33.196 g/L,8.572 g/L respectively and the experimental ε-Polylysine yield could reach 2.491±0.124 g/L on optimized medium,which agreed with the predicted value of 2.543 139 g/L and was increased by 63.6%.
出处
《食品与机械》
CSCD
北大核心
2010年第4期19-22,共4页
Food and Machinery
基金
西南科技大学青年基金(编号:07zx3125)
西南科技大学国防重点实验室基金(编号:07JGB07)
关键词
Ε-聚赖氨酸
培养基
响应面分析
白色链霉菌
ε-Polylysine
culture medium
response surface methodology
streptomyces albus