尿自然杀伤细胞检测在肾小球肾炎中的临床意义

Clinical significance in testing urinary NK cells in patients with glomerulonephritis

目的 研究尿自然杀伤（NK）细胞含量与肾小球肾炎（GN）细胞增生程度的关系.方法 用流式细胞仪（FCM）测定54例GN患者尿NK细胞含量,将肾活检结果按照是否存在细胞新月体、毛细血管内增殖分为急性增殖组30例与非急性增殖组24例,比较2组患者尿NK细胞含量.结果 30例急性增生性肾炎患者尿NK细胞含量为（14.8±3.3）% 24例非急性增生性肾炎患者尿NK细胞含量为（21.6±2.9）%,急性增生性肾炎患者尿NK细胞含量明显低于非急性增生性肾炎组（P〈0.05）.结论 NK细胞含量降低可作为间接反映GN细胞活动程度指标之一.

Objective To evaluate the clinical significance of detecting urinary natural killer（NK） cells in patients with general types of glomerulonephritis. Methods The contents of urinary NK cells from 54 patients with glomerulonephritis were measured by flowcytometry,while all patients were classified into two groups including acute proliferation group and none-acute proliferation group by renal biopsy results. The content of urinary NK cells was compared between the two groups. Results The content of urinary NK cells in acute proliferative glomerular disease group were（ 14. 8 ±3. 3）% （30 cases） ,which was significantly higher than that of（21. 6 ±2. 9）% （24 cases） in the non-acute proliferative glomerular patients（P〈0.05）. Conclusions Decreasing of the contents of NK cells in urine may be an indirect indicator of the activity of glomerulonephritis.