复方痤疮微乳膏对兔耳痤疮模型的实验研究

Experimental Study of Improvement Effect of Compound Acne Micro-emulsion Cream on Rabbit Ear Acne Model

目的:考察复方痤疮微乳膏(主要成分为阿奇霉素、螺内酯、维A酸)对兔耳痤疮模型皮损的改善作用。方法:取成年兔20只,2只为空白对照组,18只为实验组,实验组兔双耳内侧依次涂煤焦油、表皮葡萄球菌液、煤焦油造模,造模时间28d。造模后将实验组兔分为3组,每组6只:第1组左耳为A组(高浓度复方痤疮微乳膏),右耳为B组(阿奇霉素+维A酸微乳膏);第2组左耳为C组(低浓度复方痤疮微乳膏),右耳为D组(维A酸乳膏);第3组左耳为E组(护手霜),分别涂药,药量均为(0.5±0.05)g·d-1,连续给药14d,并设第3组右耳为F组(不用药)即模型对照,观察涂药前、后兔耳皮损情况,并进行组织学评价。结果:与空白对照组比较,实验兔耳均成功建立痤疮模型;与涂药前比较,涂药后皮损情况A、C组基本恢复正常,B、D组显著改善,E组有部分改善,F组明显加重;与B、D组比较,A、C组有显著性差异(P<0.05或P<0.01)。结论:复方痤疮微乳膏对兔耳痤疮模型皮损的改善作用显著。

OBJECTIVE： To study the improvement effect of Compound acne micro-emulsion cream （contain azithromycin, spirolactone and tretinoin） on skin lesion in ear of acne model rabbits. METHODS： 20 adult rabbits were divided into blank control group （n=2） and experimental group （n=18）. The ears of experimental rabbits were coated with coal tar dilution, staphylococcus epidermidis liquid and coal tar for 28 d to establish acne model. Experimental rabbits were divided into 3 groups with 6 rabbits of each group： in the first group L-ear as group A （high concentration of Compound acne micro-emulsion cream） and R-ear as group B （azithromycin＋tretinoin micro-emulsion cream）; in the second group L-ear as group C （low concentration of Compound acne micro-emulsion cream） and R-ear as group D （tretinoin cream）; in the third group L-ear as group E （hand cream） with dosage of （0.5±0.05）g·d-1 for 14 day, R-ear as group F （no administration） as model control group. The conditions of ear skin lesion in rabbits were observed and histopathologic evaluation was carried out. RESULTS： Compared with blank control group, ear acne models were successfully established in all rabbits; Compared with before coating, skin lesion recovered in group A and group C basically after coating, skin lesion were improved in group B and group D significantly and in group E partially, skin lesion was aggravated in group F. Compared with group B and group D, there were significant difference in group A and group C （P0.05 or P0.01）. CONCLUSION： Compound acne micro-emulsion cream is effective in the treatment of skin lesion in ear of acne model rabbit.