HPLC法测定盐酸奈必洛尔片中主药的含量

Content Determination of Main Component in Nebivolol Hydrochloride Tablets by HPLC

目的:建立以高效液相色谱法测定盐酸奈必洛尔片中主药含量的方法。方法:色谱柱为XTerra RP18,流动相为甲醇-0.03mo·lL-1磷酸二氢钾(0.68∶0.32),流速为1.0mL·min-1,检测波长为280nm,柱温为30℃。结果:盐酸奈必洛尔检测浓度线性范围为15.24～55.88μg·mL-1(r=0.9999,n=5);平均回收率为100.3%,RSD=0.74%(n=6)。结论:本方法简便、灵敏、准确,可用于盐酸奈必洛尔片中主药的含量测定。

OBJECTIVE：To establish an HPLC method for determination the content of main component in nebivolol hydrochloride tablets. METHODS：The XTerra RP18 column was used, the mobile phase consisted of methanol-0.03 mol·L-1 KH2PO4 solution（0.68 ∶ 0.32） at a flow rate of 1.0 mL·min-1,the detection wavelength was 280 nm, and the column temperature was 30 ℃. RESULTS：The linear range of nebivolol hydrochloride was 15.24～55.88 ？g·mL-1（r=0.999 9,n=5）. The average recovery rate was 100.3% and RSD was 0.74% （n=6）.CONCLUSION：The method is simple, sensitive, accurate and can be used for the determination of nebivolol hydrochloride tablets.