尘源性炎症反应与矽肺纤维化的关系

Experimental Study on the Relationship between Inflammatory Reaction and Pulmonary Fibrosis due to Silica

目的研究不同剂量石英粉尘所致的肺内炎症反应和纤维化病变间可能的联系。方法用不同剂量的石英粉尘染尘后，对１天组和４周组大鼠分别测定其支气管肺泡灌洗液（ＢＡＬＦ）中的细胞总数和分类计数，４周组大鼠还测定了ＢＡＬＦ细胞中羟脯氨酸量（ＨＯＰ）、血清铜蓝蛋白含量（ＣＰ）和全肺胶原蛋白量，并观察肺组织的病理变化。结果随着染尘剂量增加，１天组大鼠的细胞总数和中性粒细胞数增多，４周组大鼠的细胞总数和各分类计数增多，ＨＯＰ、ＣＰ和全肺胶原蛋白量增加；４周组大鼠的炎症和纤维化各指标间有良好的相关性。结论石英所致的肺内炎症反应可能与矽肺纤维化发生发展有关。

Objective To study the relationship between inflammatory reaction and pulmonary fibrosis caused by different doses quartz.Methods Total cells(TC)and its classification in bronchoalveolar lavage fluid(BALF)were counted for the all experimental rats.Besides,the contents of hydroxyproline(HOP)in the cells of BALF,collagen in lung tissues and ceruloplasmin(CP)in plasma were determined 4 weeks later after quartz exposure,the pathological changes in the lungs were also observed.Results All the experimental rats showed that the numbers of TC and neutrophils were increased with the increasing dose,in the rats after four weeks exposure,the numbers of TC and DC,the contents of CP,HOP and collagen were also increased with the dose and there was a good correlation between the indices of inflammation and fibrosis.Conclusion The inflammation reaction caused by silica may be related to the formation and development of pulmonary fibrosis.