发酵液中谷胱甘肽含量测定方法的比较

Comparative Study on Methods for Determination of Glutathione in Fermentation Liquor

用离心法从谷胱甘肽(GSH)产生菌发酵液中分离收集菌体。分别用冻融提取法,乙醇提法和水提法从菌体中提取GSH,所得提取液中GSH含量分别用碘量法、DTNB衍生-光度法和高效液相色谱法(HPLC)测定。对3种方法所测得结果作了比较,结果表明:HPLC法测定,由于可避免共存的其他抗氧化剂和含有巯基化合物的干扰,可达到最好的准确性和选择性。因此,其测定结果与其他两种方法相比,最准确可靠。DTNB衍生-光度法的测定结果稍高但接近于HPLC的结果,其优点是比较简单,测试成本较低,适合于大规模生产的控制分析。

Bacteriotome was collected from the bacteria-producing fermentation liquor of glutathione（GSH） by centrifugation,and the GSH was extracted from the bacteriotome by the method of freeze-thaw extraction,by the method of alcohol extraction and by the method of water extraction.Content of GSH in the extract,obtained by either of the 3 above mentioned methods,was then determined by the iodometric titration,the DTNB photometric method,and the HPLC method separately.A comparative study on the results found by the 3 methods was made,and it was shown that best accuracy and selectivety was achieved by the HPLC method,as the interferences from other co-existing anti-oxidants and sulfhydryl-containing compounds were effectively eliminated in this method,and most accurate results were obtained as compared with the other 2 methods.Results obtained by the DTNB photometric method were a little bit higher but quite closer to the results of the HPLC method,and the DTNB photometric method was found to be feasible for routine analysis in large scale production.